Anti-MAPK8/9 Antibody Picoband®

SKU: PA1407

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: IHC,WB

Application Details: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse -
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Gene Name: MAPK8

Specificity: No cross reactivity with other proteins.

Background: Mitogen-activated protein kinase 8 is an enzyme that in humans is encoded by the MAPK8 gene. The protein encoded by this gene is a member of the MAP kinase family. The International Radiation Hybrid Mapping Consortium mapped the MAPK8 gene to chromosome 10. The single long open reading frame encodes a putative protein kinase with a predicted mass of 44.2 kD. This kinase play a key role in T cell proliferation, apoptosis and differentiation. The protein encoded by MAPK9 gene is also a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase targets specific transcription factors, and thus mediates immediate-early gene expression in response to various cell stimuli. It is most closely related to MAPK8, both of which are involved in UV radiation induced apoptosis, thought to be related to the cytochrome c-mediated cell death pathway. This gene and MAPK8 are also known as c-Jun N-terminal kinases. This kinase blocks the ubiquitination of tumor suppressor p53, and thus it increases the stability of p53 in nonstressed cells. Studies of this gene's mouse counterpart suggest a key role in T-cell differentiation. Several alternatively spliced transcript variants encoding distinct isoforms have been reported.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human MAPK8/9, identical to the related mouse and rat sequences.

Clonality: Polyclonal

Contents: Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Derijard B, Hibi M, Wu IH, Barrett T, Su B, Deng T, Karin M, Davis RJ (Apr 1994). JNK1: a protein kinase stimulated by UV light and Ha-Ras that binds and phosphorylates the c-Jun activation domain. Cell 76 (6): 1025–37. doi:10.1016/0092-8674(94)90380-8. PMID 8137421. 2. Gupta S, Barrett T, Whitmarsh AJ, Cavanagh J, Sluss HK, Derijard B, Davis RJ (Jul 1996). Selective interaction of JNK protein kinase isoforms with transcription factors. EMBO J 15 (11): 2760–70. PMC 450211. PMID 8654373.

Uniprot ID: P45983, P45984

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 65 kDa

Calculated Molecular Weight: 48296 MW

Gene ID: 5599, 5601

Protein Name: von Hippel-Lindau disease tumor suppressor

Gene Full Name: Mitogen-activated protein kinase 8/9

Synonyms: JNK-46 antibody; Stress-activated protein kinase 1c antibody