Anti-Lyz1/2 Antibody Picoband®

SKU: A32724

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Mouse,Rat

Applications: WB,IHC,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Mouse
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, -

Gene Name: Lyz1/2

Specificity:

Background: Lysozymes are secreted proteins that have bacteriolytic function which are critical for mammalian innate immune function. All lysozymes function to defend host animals from microbial infection by the hydrolysis of bacterial cell wall peptidoglycan. Conventional-type lysozymes (Lysozyme C) are one of three types of lysozymes; each family member is categorized based on amino acid sequence and biochemical properties. Lysozyme C is expressed in mammalian secretions like tears, urine, and milk but is also expressed by phagocytes such as macrophages, neutrophils, and dendritic cells. Lysozyme C is encoded in humans by a single LYZ gene. The mouse orthologs of Lysozyme C are encoded by two genes, Lyz1 and Lyz2, which encode Lysozyme C-1 and Lysozyme C-2 (Lysozyme C-1/2). Interestingly, Lyz2 is upregulated in microglia of Alzheimer's disease mouse model brains that have been stimulated by specific forms of activity. Lyz1 and Lyz2 are uniquely expressed in microglia, and increased Lyz2 correlates with microglia-mediated β-amyloid (Aβ) clearance, suggesting that Lysozyme C-1/2 may ly contribute to microglial-clearance of Aβ or act as a marker for certain microglial activity states in the brain.

Immunogen: E.coli-derived mouse Lyz1/2 recombinant protein (Position: K19-V148). Mouse Lyz1/2 shares 84.6% amino acid (aa) sequence identity with rat Lyz1/2.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Cortopassi, G. A., & Wilson, A. C. (1990). Recent origin of the P lysozyme gene in mice. Nucleic acids research, 18(7), 1911. 2. Gao, X., Guo, M., Zhang, Z., Shen, P., Yang, Z., & Zhang, N. (2017). Baicalin promotes the bacteriostatic activity of lysozyme on S. aureus in mammary glands and neutrophilic granulocytes in mice. Oncotarget, 8(12), 19894. 3. Cross, M., & Renkawitz, R. (1990). Repetitive sequence involvement in the duplication and divergence of mouse lysozyme genes. The EMBO journal, 9(4), 1283-1288.

Uniprot ID: P17897/P08905

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity:

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 14 kDa

Calculated Molecular Weight:

Gene ID: 17110/17105

Protein Name: Frizzled 8a protein

Gene Full Name: lysozyme 1/2

Synonyms: Lyz1; Lzp-s; Lysozyme C-1; 1,4-beta-N-acetylmuramidase C; Lysozyme C type P; Lyz2; Lyz; Lyzs; Lysozyme C-2; 1,4-beta-N-acetylmuramidase C; Lysozyme C type M