Anti-LYPLA3/PLA2G15 Antibody Picoband®

SKU: A08649

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,ICC,IF,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: PLA2G15

Specificity:

Background: LYPLA3, also known as Group XV phospholipase A2, is an enzyme that in humans is encoded by the PLA2G15 gene. Lysophospholipases are enzymes that act on biological membranes to regulate the multifunctional lysophospholipids. The protein encoded by this gene hydrolyzes lysophosphatidylcholine to glycerophosphorylcholine and a free fatty acid. This enzyme is present in the plasma and thought to be associated with high-density lipoprotein. A later paper contradicts the function of this gene. It demonstrates that this gene encodes a lysosomal enzyme instead of a lysophospholipase and has both calcium-independent phospholipase A2 and transacylase activities.

Immunogen: E.coli-derived human LYPLA3/PLA2G15 recombinant protein (Position: D92-Q372).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Abe, A., Poucher, H. K., Hiraoka, M., Shayman, J. A. Induction of lysosomal phospholipase A2 through the retinoid X receptor in THP-1 cells. J. Lipid Res. 45: 667-673, 2004. 2. Hiraoka, M., Abe, A., Shayman, J. A. Cloning and characterization of a lysosomal phospholipase A2, 1-O-acylceramide synthase. J. Biol. Chem. 277: 10090-10099, 2002. 3. Taniyama, Y., Shibata, S., Kita, S., Horikoshi, K., Fuse, H., Shirafuji, H., Sumino, Y., Fujino, M. Cloning and expression of a novel lysophospholipase which structurally resembles lecithin cholesterol acyltransferase. Biochem. Biophys. Res. Commun. 257: 50-56, 1999.

Uniprot ID: Q8NCC3

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 42-57 kDa

Calculated Molecular Weight: 36881 MW

Gene ID: 23659

Protein Name: Lysosomal phospholipase A and acyltransferase

Gene Full Name: phospholipase A2 group XV

Synonyms: 1 O acylceramide synthase, ACS, Group XV phospholipase A2, GXVPLA2, LCAT like lysophospholipase, LLPL, LPLA2, LYPLA3, Lysophospholipase 3, Lysosomal phospholipase A2, phospholipase A2, group XV, PLA2G15