Anti-LGI4 Antibody Picoband®

SKU: A12305-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: ELISA,IF,IHC,ICC,WB,Flow Cytometry

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: LGI4

Specificity:

Background: The leucine-rich, glioma inactivated gene 4 (LGI4) is a member of the LGI family. The LGI family consists of four of highly related proteins containing leucine-rich repeats (LRRs). Despite its high homology with LGI1 and similar pattern of expression, mutations in LGI4 have not been found to be associated with autodominant lateral temporal epilepsy (ADTLE). However, the LGI4 gene is located in a region linked to benign familial infantile convulsions and a GC-to-AT polymorphism in LGI4 was correlated with childhood absence epilepsy. Other studies showed that decreasing LGI4 expression in cultured cells inhibits myelination, indicating that LGI4 may play a role in neural development.

Immunogen: E.coli-derived human LGI4 recombinant protein (Position: E225-H531).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Bermingham, J. R., Jr., Shearin, H., Pennington, J., O'Moore, J., Jaegle, M., Driegen, S., van Zon, A., Darbas, A., Ozkaynak, E., Ryu, E. J., Milbrandt, J., Meijer, D. The claw paw mutation reveals a role for Lgi4 in peripheral nerve development. Nature Neurosci. 9: 76-84, 2006. 2. Gu, W., Wevers, A., Schroder, H., Grzeschik, K.-H., Derst, C., Brodtkorb, E., de Vos, R., Steinlein, O. K. The LGI1 gene involved in lateral temporal lobe epilepsy belongs to a new subfamily of leucine-rich repeat proteins. FEBS Lett. 519: 71-76, 2002. 3. Senechal, K. R., Thaller, C., Noebels, J. L. ADPEAF mutations reduce levels of secreted LGI1, a putative tumor suppressor protein linked to epilepsy. Hum. Molec. Genet. 14: 1613-1620, 2005.

Uniprot ID: Q8N135

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross reactivity with other proteins.

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 59 kDa

Calculated Molecular Weight: 52588 MW

Gene ID: 163175

Protein Name: Leucine-rich repeat LGI family member 4

Gene Full Name: leucine rich repeat LGI family member 4

Synonyms: LGI4; LGIL3; UNQ6515/PRO21485; Leucine-rich repeat LGI family member 4; LGI1-like protein 3; Leucine-rich glioma-inactivated protein 4