Anti-LEMD3 Antibody Picoband®

SKU: A04957-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: LEMD3

Specificity:

Background: LEM domain-containing protein 3 (LEMD3), also known as MAN1, is an integral protein in the inner nuclear membrane (INM) of the nuclear envelope. It is encoded by the LEMD3 gene[5] and was first identified after it was isolated from the serum of a patient with a collagen vascular disease. This locus encodes a LEM domain-containing protein. The encoded protein functions to antagonize transforming growth factor-beta signaling at the inner nuclear membrane. Two transcript variants encoding different isoforms have been found for this gene. Mutations in this gene have been associated with osteopoikilosis, Buschke-Ollendorff syndrome and melorheostosis.

Immunogen: E.coli-derived human LEMD3 recombinant protein (Position: H535-H852).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Butkus, C. E., Michels, V. V., Lindor, N. M., Cooney, W. P., III. Melorheostosis in a patient with familial osteopoikilosis. Am. J. Med. Genet. 72: 43-46, 1997. 2. Debeer, P., Pykels, E., Lammens, J., Devriendt, K., Fryns, J.-P. Melorheostosis in a family with autosomal dominant osteopoikilosis: report of a third family. Am. J. Med. Genet. 119A: 188-193, 2003. 3. Hellemans, J., Preobrazhenska, O., Willaert, A., Debeer, P., Verdonk, P. C. M., Costa, T., Janssens, K., Menten, B., Van Roy, N., Vermeulen, S. J. T., Savarirayan, R., Van Hul, W., and 9 others. Loss-of-function mutations in LEMD3 result in osteopoikilosis, Buschke-Ollendorff syndrome and melorheostosis. Nature Genet. 36: 1213-1218, 2004.

Uniprot ID: Q9Y2U8

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross reactivity with other proteins.

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 120 kDa

Calculated Molecular Weight: 52588 MW

Gene ID: 23592

Protein Name: Inner nuclear membrane protein Man1

Gene Full Name: LEM domain containing 3

Synonyms: MAN1; MAN1; Inner nuclear membrane protein Man1; LEM domain-containing protein 3