Anti-Laminin LAMA1 Monoclonal Antibody

SKU: MA1054

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Pig

Applications: IHC

Application Details: Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, feline, pig

Gene Name: LAMA1; LAMA2; LAMB1; LAMB2

Specificity: No cross reactivity with other proteins.

Background: Laminin is a heterotrimeric extracellular matrix protein consisting of 3 chains: alpha-1,beta-1 and gamma-1, formerly called beta-2 (LAMA2). This gene is over 260,000 base pairs and contains 64 exons. Laminin is similar with merosin, a basement membrane-associated protein found in placenta, striated muscle, and peripheral nerve, and both of them are members of the same family of basement membrane proteins. And merosin is the same as laminin M, a striated muscle-specific, basal-lamina-associated protein, it may play a primary role in the pathogenesis of that disorder.

Immunogen: Human laminin.

Clonality: Monoclonal

Contents: Mouse IgG in stabilizing components, 1.2% sodium acetate and 0.01mg NaN3. *This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Purification: Ascites

Reconstitution: Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Reference: 1. Zhang, X.; Vuolteenaho, R.; Tryggvason, K. : Structure of the human laminin alpha-2-chain gene (LAMA2), which is affected in congenital muscular dystrophy. J. Biol. Chem. 271: 27664-27669, 1996. 2. Ehrig, K.; Leivo, I.; Argraves, W. S.; Ruoslahti, E.; Engvall, E. : Merosin, a tissue-specific basement membrane protein, is a laminin-like protein. Proc. Nat. Acad. Sci. 87: 3264-3268, 1990. 3. Arahata, K.; Hayashi, Y. K.; Mizuno, Y.; Yoshida, M.; Ozawa, E. : Dystrophin-associated glycoprotein and dystrophin co-localisation at sarcolemma in Fukuyama congenital muscular dystrophy. (Letter) Lancet 342: 623-624, 1993.

Uniprot ID: P15800

Host: Mouse

Concentration: Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Mouse IgG1

Phospho_site:

Clone Number: Clone: LAM-89

Observed Molecular Weight: 68 kDa

Calculated Molecular Weight: 196474 MW

Gene ID: Human(284217), Mouse(16772), Rat(316758), Human(3908), Mouse(16773), Rat(309368), Human(3912), Mouse(16777), Rat(298941), Human(3913), Mouse(16779), Rat(25473)

Protein Name: aldehyde dehydrogenase (NAD(+))

Gene Full Name: laminin subunit beta 2

Synonyms: LAMA; Laminin a; LAMM; CLM; LIS5; LAMB1; LAMS; NPHS5; Laminin M chain; Laminin-12 subunit alpha; Laminin-2 subunit alpha; Laminin-4 subunit alpha; Merosin heavy chain; S-LAM beta