Anti-Klk7 Antibody Picoband®

SKU: A02739-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Rat

Applications: WB,IHC,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Rat
ELISA, 0.1-0.5 μg/ml, Rat

Gene Name: Klk7

Specificity:

Background: Kallikrein-related peptidase 7 (KLK7) is a serine protease that in humans is encoded by the KLK7 gene. KLK7 is a chymotrypsin-like serine protease of the member of peptidase S1 family. It is synthesized with a signal peptide of 22 amino acids (aa 1-22) and a propeptide region (aa 23-29) that are subsequently cleaved to generate the mature form. Human KLK7 displays about 40 to 60% amino acid homology with other members of the family. It cleaves proteins with aromatic side chains in the P1 position. KLK7 is abundantly expressed in the skin and is also present in the brain, mammary gland, cerebellum, spinal cord, and kidney. Lower levels of expression have been reported in in salivary glands, uterus, thymus, thyroid, placenta, trachea, and testis. Its levels are shown to be up-regulated in ovarian carcinoma, especially late-stage serous carcinoma. Less differentiated tumors of more advanced stage show higher level KLK7 expression. KLK7 activity is inhibited by zinc and copper ions

Immunogen: E.coli-derived rat Klk7 recombinant protein (Position: K30-N238).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Egelrud, T. Purification and preliminary characterization of stratum corneum chymotryptic enzyme: a proteinase that may be involved in desquamation. J. Invest. Derm. 101: 200-204, 1993. 2. Gan, L., Lee, I., Smith, R., Argonza-Barrett, R., Lei, H., McCuaig, J., Moss, P., Paeper, B., Wang, K. Sequencing and expression analysis of the serine protease gene cluster located in chromosome 19q13 region. Gene 257: 119-130, 2000. 3. Hansson, L., Stromqvist, M., Backman, A., Wallbrandt, P., Carlstein, A., Egelrud, T. Cloning, expression, and characterization of stratum corneum chymotryptic enzyme: a skin-specific human serine proteinase. J. Biol. Chem. 269: 19420-19426, 1994.

Uniprot ID: P36373

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 38 kDa

Calculated Molecular Weight: 12252 MW

Gene ID: 292852

Protein Name: Glandular kallikrein-7, submandibular/renal

Gene Full Name: kallikrein 1

Synonyms: Klk7; Klk-7; Glandular kallikrein-7, submandibular/renal; rGK-7; Esterase B; Kallikrein-related protein K1; Proteinase A; RSKG-7; Tissue kallikrein