Anti-ITCH/AIP4 Antibody Picoband® (monoclonal, 5E12)

SKU: M00195-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,Flow Cytometry

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x⁶ cells, Human

Gene Name: ITCH

Specificity:

Background: ITCH is an ubiquitin-conjugating enzyme. This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein plays a role in multiple cellular processes including erythroid and lymphoid cell differentiation and the regulation of immune responses. Mutations in this gene are a cause of syndromic multisystem autoimmune disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Immunogen: E.coli-derived human ITCH/AIP4 recombinant protein (Position: K17-E358).

Clonality: Monoclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Dorjbal B; Derse D; Lloyd P; Soheilian F; Nagashima K; Heidecker G. “The role of ITCH protein in human T-cell leukemia virus type 1 release”. J Biol Chem, 2011 Sep 9. 2. Rossi M; Inoue S; Walewska R; Knight RA; Dyer MJ; Cohen GM; Melino G. “Caspase cleavage of Itch in chronic lymphocytic leukemia cells”. Biochem Biophys Res Commun, 2009 Feb 13.

Uniprot ID: Q96J02

Host: Mouse

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Mouse IgG2b

Phospho_site:

Clone Number: Clone: 5E12

Observed Molecular Weight: 103 kDa

Calculated Molecular Weight: 46659 MW

Gene ID: 83737

Protein Name: E3 ubiquitin-protein ligase Itchy homolog

Gene Full Name: itchy E3 ubiquitin protein ligase

Synonyms: AIF4; AIP4; ITCH; NAPP1; NFE2 associated polypeptide 1