Anti-IRGM/Irgm1 Antibody Picoband®

SKU: A33515

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Mouse

Applications: WB,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Mouse
ELISA, 0.1-0.5 μg/ml, -

Gene Name: Irgm1

Specificity:

Background: Immunity-related GTPase family M protein (IRGM), also known as interferon-inducible protein 1 (IFI1), is an enzyme that in humans is IRGM gene. This gene encodes a member of the p47 immunity-related GTPase family. The encoded protein may play a role in the innate immune response by regulating autophagy formation in response to intracellular pathogens. Polymorphisms that affect the normal expression of this gene are associated with a susceptibility to Crohn's disease and tuberculosis. Alternative splicing results in multiple transcript variants encoding different isoforms.

Immunogen: E.coli-derived mouse IRGM/Irgm1 recombinant protein (Position: E50-K333).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Amre, D. K., Mack, D. R., Morgan, K., Krupoves, A., Costea, I., Lambrette, P., Grimard, G., Dong, J., Feguery, H., Bucionis, V., Deslandres, C., Levy, E., Seidman, E. G. Autophagy gene ATG16L1 but not IRGM is associated with Crohn's disease in Canadian children. Inflamm. Bowel Dis. 15: 501-507, 2009. 2. Bekpen, C., Hunn, J. P., Rohde, C., Parvanova, I., Guethlein, L., Dunn, D. M., Glowalla, E., Leptin, M., Howard, J. C. The interferon-inducible p47 (IRG) GTPases in vertebrates: Loss of the cell autonomous resistance mechanism in the human lineage. Genome Biol. 6: R92, 2005. Note: Electronic Article. 3. Brest, P., Lapaquette, P., Souidi, M., Lebrigand, K., Cesaro, A., Vouret-Craviari, V., Mari, B., Barbry, P., Mosnier, J.-F., Hebuterne, X., Harel-Bellan, A., Mograbi, B., Darfeuille-Michaud, A., Hofman, P. A synonymous variant in IRGM alters a binding site for miR-196 and causes deregulation of IRGM-dependent xenophagy in Crohn's disease. Nature Genet. 43: 242-245, 2011.

Uniprot ID: Q60766

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 45 kDa

Calculated Molecular Weight: 14377 MW

Gene ID: 15944

Protein Name: Immunity-related GTPase family M protein 1

Gene Full Name: immunity-related GTPase family M member 1

Synonyms: Ifi1; Iigp3; Irgm; Irgm1; LRG-47; Interferon-inducible protein 1; Interferon-inducible GTPase 3