Anti-Hephaestin/HEPH Antibody Picoband®

SKU: A05316-1

Size: 100 μg

Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Mouse
ELISA, 0.1-0.5 μg/ml

Gene Name: HEPH

Specificity:

Background: Enables ferroxidase activity. Involved in intestinal iron absorption; multicellular organismal-level iron ion homeostasis; and positive regulation of iron export across plasma membrane. Acts upstream of or within erythrocyte differentiation. Predicted to be located in perinuclear region of cytoplasm. Predicted to be active in basolateral plasma membrane. Orthologous to human HEPH (hephaestin).

Immunogen: E.coli-derived human Hephaestin/HEPH recombinant protein (Position: E348-Q1157). Mouse Hephaestin/HEPHshares 85.6% and 96.2% amino acid (aa) sequence identity with human and rat Hephaestin/HEPH, respectively.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Anderson, G. J., Murphy, T. L., Cowley, L., Evans, B. A., Halliday, J. W., McLaren, G. D. Mapping the gene for sex-linked anemia: an inherited defect of intestinal iron absorption in the mouse. Genomics 48: 34-39, 1998. 2. Bannerman, R. M. Genetic defects of iron transport. Fed. Proc. 35: 2281-2285, 1976. 3. Edwards, J. A., Hoke, J. E., Mattioli, M., Reichlin, M. Ferritin distribution and synthesis in sex-linked anemia. J. Lab. Clin. Med. 90: 68-76, 1977.

Uniprot ID: Q9Z0Z4

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 130 kDa

Calculated Molecular Weight: 130 kDa

Gene ID: 15203

Protein Name: Hephaestin

Gene Full Name: hephaestin

Synonyms: CPL; HEPH; Hephaestin; KIAA0698