Anti-Hepatocyte growth factor HGF Antibody Picoband®

SKU: RP1062

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB

Application Details: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Gene Name: HGF

Specificity: No cross reactivity with other proteins.

Background: Hepatocyte growth factor/scatter factor (HGF/SF) is a paracrine cellular growth, motility and morphogenic factor. This gene is mapped to 7q21.11. It is secreted by mesenchymal cells and targets and acts primarily uponepithelial cells and endothelial cells, but also acts on haemopoietic progenitor cells. It has been shown to have a major role in embryonic organ development, specifically in myogenesis, in adult organ regeneration and in wound healing. HGF can regulate cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. HGF also serves as a paracrine mediator to control placental development and growth. HGF was identified as one of the liver sinusoidal endothelial cell-derived paracrine mediators promoting hepatocyte growth.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of mouse HGF, different from the related human sequence by five amino acids, and from the related rat sequence by one amino acid.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Bottaro DP, Rubin JS, Faletto DL, Chan AM, Kmiecik TE, Vande Woude GF, Aaronson SA (February 1991). Identification of the hepatocyte growth factor receptor as the c-met proto-oncogene product. Science251 (4995): 802–4. 2. Gallagher, J.T., Lyon, M. (2000). Molecular structure of Heparan Sulfate and interactions with growth factors and morphogens. In Iozzo, M, V. Proteoglycans: structure, biology and molecular interactions. Marcel Dekker Inc. New York, New York. pp. 27–59. 3. LeCouter, J., Moritz, D. R., Li, B., Phillips, G. L., Liang, X. H., Gerber, H.-P., Hillan, K. J., Ferrara, N. Angiogenesis-independent endothelial protection of liver: role of VEGFR-1. Science 299: 890-893, 2003.

Uniprot ID: Q08048

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 34 kDa

Calculated Molecular Weight: 82945 MW

Gene ID: 15234

Protein Name: Hepatocyte growth factor

Gene Full Name: hepatocyte growth factor

Synonyms: F TCF; Hepatocyte growth factor; Hepatopoeitin A; HGF; HGFB; HPTA; Scatter factor; SF