Anti-HEF1/NEDD9 Antibody Picoband®

SKU: PB9289

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Monkey,Mouse,Rat

Applications: Flow Cytometry,IF,IHC,ICC,WB

Application Details: Western blot, 0.1-0.5μg/ml, Human, Monkey, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human

Gene Name: NEDD9

Specificity: No cross reactivity with other proteins.

Background: Enhancer of filamentation 1 (EF1), also known as NEDD-9, is a protein that in humans is encoded by the NEDD9 gene. The protein encoded by this gene is a member of the CRK-associated substrates family. Members of this family are adhesion docking molecules that mediate protein-protein interactions for signal transduction pathways. It is mapped to 6p24.2. This protein is a focal adhesion protein that acts as a scaffold to regulate signaling complexes important in cell attachment, migration and invasion as well as apoptosis and the cell cycle. This protein has also been reported to have a role in cancer metastasis. NEDD9 is also a highly relevant cancer gene that governs metastatic potential in human melanoma. What’s more, it has been speculated that NEDD9 may be an important linking element between extracellular signaling and regulation of the cytoskeleton.

Immunogen: E.coli-derived human HEF1 recombinant protein (Position: K273-E421). Human HEF1 shares 83% amino acid (aa) sequence identity with mouse HEF1.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Law, S. F., Estojak, J., Wang, B., Mysliwiec, T., Kruh, G., Golemis, E. A. Human enhancer of filamentation 1, a novel p130(cas)-like docking protein, associates with focal adhesion kinase and induces pseudohyphal growth in Saccharomyces cerevisiae. Molec. Cell. Biol. 16: 3327-3337, 1996. 2. Gross, M. B. Personal Communication. Baltimore, Md. 9/4/2012.

Uniprot ID: Q14511

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 93-105 kDa

Calculated Molecular Weight: 92861 MW

Gene ID: 4739

Protein Name: Enhancer of filamentation 1

Gene Full Name: neural precursor cell expressed, developmentally down-regulated 9

Synonyms: neural precursor cell expressed; developmentally down-regulated 9; CAS-L; CAS2; CASL; CASS2; HEF1; NEDD9