Anti-HCRT Antibody

SKU: A01633-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Mouse,Rat

Applications: IF,IHC

Application Details: Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Mouse, Rat
Immunofluorescence, 5 μg/ml, Mouse, Rat

Gene Name: HCRT

Specificity:

Background: This gene encodes a hypothalamic neuropeptide precursor protein that gives rise to two mature neuropeptides, orexin A and orexin B, by proteolytic processing. Orexin A and orexin B, which bind to orphan G-protein coupled receptors HCRTR1 and HCRTR2, function in the regulation of sleep and arousal. This neuropeptide arrangement may also play a role in feeding behavior, metabolism, and homeostasis.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human HCRT, identical to the related mouse and rat sequences.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Adamantidis, A. R., Zhang, F., Aravanis, A. M., Deisseroth, K., de Lecea, L. Neural substrates of awakening probed with optogenetic control of hypocretin neurons. Nature 450: 420-424, 2007. 2. Arii, J., Kanbayashi, T., Tanabe, Y., Sawaishi, Y., Kimura, S., Watanabe, A., Mishima, K., Hishikawa, Y., Shimizu, T., Nishino, S. CSF hypocretin-1 (orexin-A) levels in childhood narcolepsy and neurologic disorders. Neurology 63: 2440-2442, 2004. 3. Blouin, A. M., Thannickal, T. C., Worley, P. F., Baraban, J. M., Reti, I. M., Siegel, J. M. Narp immunostaining of human hypocretin (orexin) neurons: loss in narcolepsy. Neurology 65: 1189-1192, 2005.

Uniprot ID: O43612

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross reactivity with other proteins.

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 100,150 kDa

Calculated Molecular Weight: 52588 MW

Gene ID: 3060

Protein Name: Hypocretin neuropeptide precursor

Gene Full Name: hypocretin neuropeptide precursor

Synonyms: Orexin; Hypocretin; Hcrt; Orexin-A; Hypocretin-1; Hcrt1; Orexin-B; Hypocretin-2; Hcrt2; HCRT; OX; PPORX; PPOX