Anti-Guanylate-binding protein 1 GBP1 Antibody Picoband®

SKU: PA1550

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human

Applications: WB

Application Details: Western blot, 0.1-0.5μg/ml, Human

Gene Name: GBP1

Specificity: No cross reactivity with other proteins.

Background: Interferons induce a large number of genes in their target cells, including those coding for guanylate-binding proteins (GBPs). GBPs, such as GBP1, are characterized by their ability to specifically bind guanine nucleotides (GMP, GDP, and GTP) and are distinguished from the GTP-binding proteins by the presence of 2 binding motifs rather than 3. The 593-amino acid GBP1 protein shares 77% and 88% identity with GBP2 and GBP3, respectively. All GBPs, including GBP1, have a conserved N-terminal globular GTP-binding domain containing 2 consensus sequences and a third T (L/V)RD sequence not found in other GTPases. The GBP1 gene to the GBP gene cluster on chromosome 1p22.2. It is located telomeric to GBP2 and centromeric to GBP3. High expression of GBP1, GBP2, and GBP3 are in endothelial cells after stimulation with IFNG, TNF, or IL1B .

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human GBP1.

Clonality: Polyclonal

Contents: Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Cheng, Y.-S. E., Patterson, C. E., Staeheli, P.Interferon-induced guanylate-binding proteins lack an N(T)KXD consensus motif and bind GMP in addition to GDP and GTP.Molec. Cell. Biol. 11: 4717-4725, 1991. 2. Olszewski, M. A., Gray, J., Vestal, D. J.In silico genomic analysis of the human and murine guanylate-binding protein (GBP) gene clusters.J. Interferon Cytokine Res. 26: 328-352, 2006. 3. Tripal, P., Bauer, M., Naschberger, E., Mortinger, T., Hohenadl, C., Cornali, E., Thurau, M., Sturzl, M.Unique features of different members of the human guanylate-binding protein family.J. Interferon Cytokine Res. 27: 44-52, 2007.

Uniprot ID: P32455

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 68 kDa

Calculated Molecular Weight: 67931 MW

Gene ID: 2633

Protein Name: Guanylate-binding protein 1

Gene Full Name: guanylate binding protein 1

Synonyms: GBP 1; GBP1; GTP binding protein 1; HuGBP 1