Anti-GRO alpha/CXCL1 Antibody Picoband®

SKU: PA1760

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human

Applications: WB

Application Details: Western blot, 0.1-0.5μg/ml, Human

Gene Name: CXCL1

Specificity: No cross reactivity with other proteins.

Background: CXCL1 (Chemokine, CXC motif, Ligand 1), also called GRO1, SCYB1, GROA or MGSA, is a small cytokine belonging to the CXC chemokine family that was previously called GRO1 oncogene, GROalpha, KC, Neutrophil-activating protein 3 (NAP-3) and melanoma growth stimulating activity, alpha (MSGA-alpha). In humans, this protein is encoded by the CXCL1 gene. The CXCL1 gene is mapped on 4q13.3. CXCL1 is secreted by human melanoma cells, has mitogenic properties and is implicated in melanoma pathogenesis. CXCL1 is expressed by macrophages, neutrophils and epithelial cells, and has neutrophil chemoattractant activity. CXCL1 plays a role in spinal cord development by inhibiting the migration of oligodendrocyte precursors and is involved in the processes of angiogenesis, inflammation,wound healing, and tumorigenesis. Signaling through Cxcr2, Cxcl1 inhibited oligodendrocyte precursor migration. The migrational arrest was rapid, reversible, and concentration dependent, and it reflected enhanced cell/substrate interactions. White matter expression of Cxcl1 was temporospatially regulated. Others contribute to aggressive growth selectivity in the lung. Among the lung metastasis signature genes identified, several, including CXCL1, were functionally validated.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human GRO alpha.

Clonality: Polyclonal

Contents: Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Anisowicz, A., Zajchowski, D., Stenman, G., Sager, R. Functional diversity of GRO gene expression in human fibroblasts and mammary epithelial cells. Proc. Nat. Acad. Sci. 85: 9645-9649, 1988. 2. Horuk, R., Yansura, D. G., Reilly, D., Spencer, S., Bourell, J., Henzel, W., Rice, G., Unemori, E. Purification, receptor binding analysis, and biological characterization of human melanoma growth stimulating activity (MGSA): evidence for a novel MGSA receptor. J. Biol. Chem. 268: 541-546, 1993. 3. O'Donovan, N., Galvin, M., Morgan, J. G. Physical mapping of the CXC chemokine locus on human chromosome 4. Cytogenet. Cell Genet. 84: 39-42, 1999.

Uniprot ID: P09341

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 11 kDa

Calculated Molecular Weight: 11301 MW

Gene ID: 2919

Protein Name: Growth-regulated alpha protein

Gene Full Name: C-X-C motif chemokine ligand 1

Synonyms: C X C motif chemokine 1; CXCL1; FSP; GRO; GRO alpha(1 73); GRO1; GROa; Growth regulated alpha protein; MGSA; MGSA a; NAP 3; SCYB1