Anti-GRID2 Antibody Picoband®

SKU: A05305-2

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,Flow Cytometry

Application Details: Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human, Rat

Gene Name: GRID2

Specificity:

Background: Glutamate receptor, ionotropic, delta 2, also known as GluD2, GluRδ2, or δ2, is a protein that in humans is encoded by the GRID2 gene. The protein encoded by this gene is a member of the family of ionotropic glutamate receptors which are the predominant excitatory neurotransmitter receptors in the mammalian brain. The encoded protein is a multi-pass membrane protein that is expressed selectively in cerebellar Purkinje cells. A point mutation in the mouse ortholog, associated with the phenotype named 'lurcher', in the heterozygous state leads to ataxia resulting from selective, cell-autonomous apoptosis of cerebellar Purkinje cells during postnatal development. Mice homozygous for this mutation die shortly after birth from massive loss of mid- and hindbrain neurons during late embryogenesis. This protein also plays a role in synapse organization between parallel fibers and Purkinje cells. Alternate splicing results in multiple transcript variants encoding distinct isoforms. Mutations in this gene cause cerebellar ataxia in humans.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human GRID2, identical to the related mouse and rat sequences.

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Araki, K., Meguro, H., Kushiya, E., Takayama, C., Inoue, Y., Mishina, M. Selective expression of the glutamate receptor channel delta-2 subunit in cerebellar Purkinje cells. Biochem. Biophys. Res. Commun. 197: 1267-1276, 1993. 2. Hills, L. B., Masri, A., Konno, K., Kakegawa, W., Lam, A.-T. N., Lim-Melia, E., Chandy, N., Hill, R. S., Partlow, J. N., Al-Saffar, M., Nasir, R., Stoler, J. M., Barkovich, A. J., Watanabe, M., Yuzaki, M., Mochida, G. H. Deletions in GRID2 lead to a recessive syndrome of cerebellar ataxia and tonic upgaze in humans. Neurology 81: 1378-1386, 2013. 3. Hirai, H., Launey, T., Mikawa, S., Torashima, T., Yanagihara, D., Kasaura, T., Miyamoto, A., Yuzaki, M. New role of delta-2-glutamate receptors in AMPA receptor trafficking and cerebellar function. Nature Neurosci. 6: 869-876, 2003.

Uniprot ID: O43424

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 113 kDa

Calculated Molecular Weight: 39411 MW

Gene ID: 2895

Protein Name: Glutamate receptor ionotropic, delta-2

Gene Full Name: glutamate ionotropic receptor delta type subunit 2

Synonyms: glutamate ionotropic receptor delta type subunit 2; GluD2; SCAR18; GRID2