Anti-GPT2 Antibody Picoband®

SKU: A07547-1

Size: 100 μg

Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,ICC/IF,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml

Gene Name: GPT2

Specificity:

Background: This gene encodes a mitochondrial alanine transaminase, a pyridoxal enzyme that catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate. Alanine transaminases play roles in gluconeogenesis and amino acid metabolism in many tissues including skeletal muscle, kidney, and liver. Activating transcription factor 4 upregulates this gene under metabolic stress conditions in hepatocyte cell lines. A loss of function mutation in this gene has been associated with developmental encephalopathy. Alternative splicing results in multiple transcript variants.

Immunogen: E.coli-derived human GPT2 recombinant protein (Position: Q56-A523). Human GPT2 shares 94.4% amino acid (aa) sequence identity with mouse GPT2.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Celis, K., Shuldiner, S., Haverfield, E. V., Cappell, J., Yang, R., Gong, D.-W., Chung, W. K. Loss of function mutation in glutamic pyruvate transaminase 2 (GPT2) causes developmental encephalopathy. J. Inherit. Metab. Dis. 38: 941-948, 2015. 2. Hengel, H., Keimer, R., Deigendesch, W., Riess, A., Marzouqa, H., Zaidan, J., Bauer, P., Schols, L. GPT2 mutations cause developmental encephalopathy with microcephaly and features of complicated hereditary spastic paraplegia. Clin. Genet. 94: 356-361, 2018. 3. Kaymakcalan, H., Yarman, Y., Goc, N., Toy, F., Meral, C., Ercan-Sencicek, A. G., Gunel, M. Novel compound heterozygous mutations in GPT2 linked to microcephaly, and intellectual developmental disability with or without spastic paraplegia. Am. J. Med. Genet. 176A: 421-425, 2018.

Uniprot ID: Q8TD30

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 58 kDa

Calculated Molecular Weight: 58 kDa

Gene ID: 84706

Protein Name: Alanine aminotransferase 2

Gene Full Name: glutamic--pyruvic transaminase 2

Synonyms: ALAT2; Alanine aminotransferase 2; Glutamic--pyruvic transaminase 2; Glutamic--alanine transaminase 2; EC:2.6.1.2