Anti-Glypican 5/GPC5 Antibody Picoband®

SKU: A07532-1

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human

Applications: WB,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5μg/ml, Human
Immunocytochemistry/Immunofluorescence, 4μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human
ELISA, 0.1-0.5μg/ml, -

Gene Name: GPC5

Specificity:

Background: Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation.

Immunogen: E.coli-derived human Glypican 5/GPC5 recombinant protein (Position: Q28-D538).

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Neat, M. J., Foot, N., Jenner, M., Goff, L., Ashcroft, K., Burford, D., Dunham, A., Norton, A., Lister, T. A., Fitzgibbon, J. Localisation of a novel region of recurrent amplification in follicular lymphoma to an ~6.8 Mb region of 13q32-33. Genes Chromosomes Cancer 32: 236-243, 2001. 2. Veugelers, M., Vermeesch, J., Reekmans, G., Steinfeld, R., Marynen, P., David, G. Characterization of glypican-5 and chromosomal localization of human GPC5, a new member of the glypican gene family. Genomics 40: 24-30, 1997. 3. Yu, W., Inoue, J., Imoto, I., Matsuo, Y., Karpas, A., Inazawa, J. GPC5 is a possible target for the 13q31-q32 amplification detected in lymphoma cell lines. J. Hum. Genet. 48: 331-335, 2003.

Uniprot ID: P78333

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 64 kDa

Calculated Molecular Weight: 157578 MW

Gene ID: 2262

Protein Name: Glypican-5 [Cleaved into: Secreted glypican-5]

Gene Full Name: glypican 5

Synonyms: Glypican-5; Secreted glypican-5; GPC5