Anti-Glycogen synthase 1/GYS1 Antibody Picoband®

SKU: A03512-3

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: ELISA,Flow Cytometry,IF,IHC,ICC,WB

Application Details: Western blot, 0.1-0.25 μg/ml, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: GYS1

Specificity:

Background: Glycogen synthase (UDP-glucose-glycogen glucosyltransferase) is a key enzyme in glycogenesis, the conversion of glucose into glycogen. The protein encoded by this gene catalyzes the addition of glucose monomers to the growing glycogen molecule through the formation of alpha-1,4-glycoside linkages. Mutations in this gene are associated with muscle glycogen storage disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Immunogen: E.coli-derived human Glycogen synthase 1/GYS1 recombinant protein (Position: D60-A640).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Browner, M. F., Nakano, K., Bang, A. G., Fletterick, R. J. Human muscle glycogen synthase cDNA sequence: a negatively charged protein with an asymmetric charge distribution. Proc. Nat. Acad. Sci. 86: 1443-1447, 1989. 2. Cameron, J. M., Levandovskiy, V., MacKay, N., Utgikar, R., Ackerley, C., Chiasson, D., Halliday, W., Raiman, J., Robinson, B. H. Identification of a novel mutation in GYS1 (muscle-specific glycogen synthase) resulting in sudden cardiac that, that is diagnosable from skin fibroblasts. Molec. Genet. Metab. 98: 378-382, 2009. 3. Groop, L. C., Kankuri, M., Schalin-Jantti, C., Ekstrand, A., Nikula-Ijas, P., Widen, E., Kuismanen, E., Eriksson, J., Franssila-Kallunki, A., Saloranta, C., Koskimies, S. Association between polymorphism of the glycogen synthase gene and non-insulin-dependent diabetes mellitus. New Eng. J. Med. 328: 10-14, 1993. Note: Erratum: New Eng. J. Med. 328: 1136 only, 1993.

Uniprot ID: P13807

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 84-90 kDa

Calculated Molecular Weight:

Gene ID: 2997

Protein Name: Glycogen [starch] synthase, muscle

Gene Full Name: glycogen synthase 1

Synonyms: glycogen synthase 1 (muscle); GSY; GYS; GYS1