Anti-GLUD1/2 Antibody Picoband®

SKU: A01866-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.1-0.25 μg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: GLUD1/2

Specificity:

Background: This gene encodes glutamate dehydrogenase, which is a mitochondrial matrix enzyme that catalyzes the oxidative deamination of glutamate to alpha-ketoglutarate and ammonia. This enzyme has an important role in regulating amino acid-induced insulin secretion. It is allosterically activated by ADP and inhibited by GTP and ATP. Activating mutations in this gene are a common cause of congenital hyperinsulinism. Alternative splicing of this gene results in multiple transcript variants. The related glutamate dehydrogenase 2 gene on the human X-chromosome originated from this gene via retrotransposition and encodes a soluble form of glutamate dehydrogenase. Related pseudogenes have been identified on chromosomes 10, 18 and X.

Immunogen: E.coli-derived human GLUD1/2 recombinant protein (Position: S54-A553).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Anagnou, N. P., Seuanez, H., Modi, W., O'Brien, S. J., Papmatheakis, J., Moschonas, N. Chromosomal mapping of the human glutamate dehydrogenase (GLUD) genes to chromosomes 10q21.1-21.2 and Xq26-28. (Abstract) Am. J. Hum. Genet. 45 (suppl.): A170 only, 1989. 2. Barbeau, A., Charbonneau, M., Cloutier, T. Leucocyte glutamate dehydrogenase in various hereditary ataxias. Canad. J. Neurol. Sci. 7: 421-424, 1980. 3. Colon, A. D., Plaitakis, A., Perakis, A., Berl, S., Clarke, D. D. Purification and characterization of a soluble and a particulate glutamate dehydrogenase from rat brain. J. Neurochem. 46: 1811-1819, 1986.

Uniprot ID: P00367/P49448

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 54 kDa

Calculated Molecular Weight: 50433 MW

Gene ID: 2746/2747

Protein Name: receptor protein-tyrosine kinase

Gene Full Name: glutamate dehydrogenase 1/2

Synonyms: GDH; GDH 1; GDH1; GLUD; GLUD1; glutamate dehydrogenase 1; GDH 2; GDH2; GLUD2; GLUDP1; glutamate dehydrogenase 2