Anti-GILT/IFI30 Antibody Picoband®

SKU: A05754-3

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Mouse,Rat

Applications: WB,IHC,Flow Cytometry,ELISA

Application Details: Western blot, 0.1-0.25μg/ml, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Mouse, Rat
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Mouse
ELISA, 0.1-0.5μg/ml, -

Gene Name: Ifi30

Specificity:

Background: Gamma-interferon-inducible lysosomal thiol reductase is an enzyme that, in humans, is encoded by the IFI30 gene. The protein encoded by this gene is a lysosomal thiol reductase that at low pH can reduce protein disulfide bonds. The enzyme is expressed constitutively in antigen-presenting cells and induced by gamma-interferon in other cell types. This enzyme has an important role in MHC class II-restricted antigen processing.

Immunogen: E.coli-derived mouse GILT/IFI30 recombinant protein (Position: A32-K248).

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Arunachalam, B., Phan, U. T., Geuze, H. J., Cresswell, P. Enzymatic reduction of disulfide bonds in lysosomes: characterization of a gamma-interferon-inducible lysosomal thiol reductase (GILT). Proc. Nat. Acad. Sci. 97: 745-750, 2000. 2. Maric, M., Arunachalam, B., Phan, U. T., Dong, C., Garrett, W. S., Cannon, K. S., Alfonso, C., Karlsson, L., Flavell, R. A., Cressell, P. Defective antigen processing in GILT-free mice. Science 294: 1361-1365, 2001. 3. Phan, U. T., Arunachalam, B., Cresswell, P. Gamma-interferon-inducible lysosomal thiol reductase (GILT): maturation, activity, and mechanism of action. J. Biol. Chem. 275: 25907-25914, 2000.

Uniprot ID: Q9ESY9

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 25 kDa

Calculated Molecular Weight: 16693 MW

Gene ID: 65972

Protein Name: Gamma-interferon-inducible lysosomal thiol reductase

Gene Full Name: interferon gamma inducible protein 30

Synonyms: GILT; IFI 30; IFI30; IP30