Anti-GGCX Antibody Picoband®

SKU: A02643-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse

Applications: ELISA,Flow Cytometry,IF,IHC,ICC,WB

Application Details: Western blot, 0.1-0.25 μg/ml, Human, Mouse
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: GGCX

Specificity:

Background: Gamma-glutamyl carboxylase is an enzyme that in humans is encoded by the GGCX gene, located on chromosome 2 at 2p12. This gene encodes an integral membrane protein of the rough endoplasmic reticulum that carboxylates glutamate residues of vitamin K-dependent proteins to gamma carboxyl glutamate, a modification that is required for their activity. The vitamin K-dependent protein substrates have a propeptide that binds the enzyme, with carbon dioxide, dioxide, and reduced vitamin K acting as co-substrates. Vitamin K-dependent proteins affect a number of physiologic processes including blood coagulation, prevention of vascular calcification, and inflammation. Allelic variants of this gene have been associated with pseudoxanthoma elasticum-like disorder with associated multiple coagulation factor deficiency. Alternative splicing results in multiple transcript variants.

Immunogen: E.coli-derived human GGCX recombinant protein (Position: M75-A730).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Brenner, B., Sanchez-Vega, B., Wu, S.-M., Lanir, N., Stafford, D. W., Solera, J. A missense mutation in a gamma-glutamyl carboxylase gene causes combined deficiency of all vitamin K-dependent blood coagulation factors. Blood 92: 4554-4559, 1998. 2. Brenner, B., Tavori, S., Zivelin, A., Keller, C. B., Suttie, J. W., Tatarsky, I., Seligsohn, U. Hereditary deficiency of all vitamin K-dependent procoagulants and anticoagulants. Brit. J. Haemat. 75: 537-542, 1990. 3. Kuo, W.-L., Stafford, D. W., Cruces, J., Gray, J., Solera, J. Chromosomal localization of the gamma-glutamyl carboxylase gene at 2p12. Genomics 25: 746-748, 1995.

Uniprot ID: P38435

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 88 kDa

Calculated Molecular Weight: 33310 MW

Gene ID: 2677

Protein Name: Vitamin K-dependent gamma-carboxylase

Gene Full Name: gamma-glutamyl carboxylase

Synonyms: gamma glutamyl carboxylase; GC; GGCX; VKCFD1