Anti-GGCT Antibody Picoband®

SKU: A06488-2

Size: 100 μg

Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml

Gene Name: GGCT

Specificity:

Background: The protein encoded by this gene catalyzes the formation of 5-oxoproline from gamma-glutamyl dipeptides, the penultimate step in glutathione catabolism, and may play a critical role in glutathione homeostasis. The encoded protein may also play a role in cell proliferation, and the expression of this gene is a potential marker for cancer. Pseudogenes of this gene are located on the long arm of chromosome 5 and the short arm of chromosomes 2 and 20. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Immunogen: E.coli-derived human GGCT recombinant protein (Position: N25-L188). Human GGCT shares 86.4% amino acid (aa) sequence identity with mouse GGCT.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Bissbort, S., Bender, K., Grzeschik, K. H. Assignment of the human gene for gamma-glutamyl-cyclo-transferase (GCTG) to chromosome 7p. (Abstract) Cytogenet. Cell Genet. 37: 442 only, 1984. 2. Masuda, Y., Maeda, S., Watanabe, A., Sano, Y., Aiuchi, T., Nakajo, S., Itabe, H., Nakaya, K. A novel 21-kDa cytochrome c-releasing factor is generated upon treatment of human leukemia U937 cells with geranylgeraniol. Biochem. Biophys. Res. Commun. 346: 454-460, 2006. 3. Oakley, A. J., Yamada, T., Liu, D., Coggan, M., Clark, A. G., Board, P. G. The identification and structural characterization of C7orf24 as gamma-glutamyl cyclotransferase: an essential enzyme in the gamma-glutamyl cycle. J. Biol. Chem. 283: 22031-22042, 2008. Note: Erratum: J. Biol. Chem. 283: 32152 only, 2008.

Uniprot ID: O75223

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 21 kDa

Calculated Molecular Weight: 21 kDa

Gene ID: 79017

Protein Name: Gamma-glutamylcyclotransferase

Gene Full Name: gamma-glutamylcyclotransferase

Synonyms: C7orf24; CRF21; Gamma glutamylcyclotransferase; GCTG; Ggc; GGCT