Anti-GFI1 Antibody Picoband®

SKU: A00888-3

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human
ELISA, 0.1-0.5μg/ml, -

Gene Name: GFI1

Specificity: No cross reactivity with other proteins.

Background: Zinc finger protein Gfi-1 is a transcriptional repressor that in humans is encoded by the GFI1 gene. It is mapped to 1p22.1. This gene encodes a nuclear zinc finger protein that functions as a transcriptional repressor. This protein plays a role in diverse developmental contexts, including hematopoiesis and oncogenesis. It functions as part of a complex along with other cofactors to control histone modifications that lead to silencing of the target gene promoters. Mutations in this gene cause autosomal dominant severe congenital neutropenia, and also dominant nonimmune chronic idiopathic neutropenia of adults, which are heterogeneous hematopoietic disorders that cause predispositions to leukemias and infections. Multiple alternatively spliced variants, encoding the same protein, have been identified for this gene.

Immunogen: E.coli-derived human GFI1 recombinant protein (Position: M1-K422).

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Bell, D. W., Taguchi, T., Jenkins, N. A., Gilbert, D. J., Copeland, N. G., Gilks, C. B., Zweidler-McKay, P., Grimes, H. L., Tsichlis, P. N., Testa, J. R. Chromosomal localization of a gene, GFI1, encoding a novel zinc finger protein reveals a new syntenic region between man and rodents. Cytogenet. Cell Genet. 70: 263-267, 1995. 2. Duan, Z., Horwitz, M. Targets of the transcriptional repressor oncoprotein Gfi-1. Proc. Nat. Acad. Sci. 100: 5932-5937, 2003. Note: Erratum: Proc. Nat. Acad. Sci. 108: 16134 only, 2011. 3. Gilks, C. B., Bear, S. E., Grimes, H. L., Tsichlis, P. N. Progression of interleukin-2 (IL-2)-dependent rat T cell lymphoma lines to IL-2-independent growth following activation of a gene (Gfi1) encoding a novel zinc finger protein. Molec. Cell Biol. 13: 1759-1768, 1993.

Uniprot ID: Q99684

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 55 kDa

Calculated Molecular Weight:

Gene ID: 2672

Protein Name: Zinc finger protein Gfi-1

Gene Full Name: growth factor independent 1 transcriptional repressor

Synonyms: GFI 1; GFI1; Zinc finger protein 163; Zinc finger protein Gfi 1; ZNF163