Anti-Gephyrin/GPHN Antibody Picoband®

SKU: A04560-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat,Monkey

Applications: WB,IHC,ICC,IF,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat, Monkey
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: GPHN

Specificity:

Background: Gephyrin is a protein that in humans is encoded by the GPHN gene. This gene encodes a neuronal assembly protein that anchors inhibitory neurotransmitter receptors to the postsynaptic cytoskeleton via high affinity binding to a receptor subunit domain and tubulin dimers. In nonneuronal tissues, the encoded protein is also required for molybdenum cofactor biosynthesis. Mutations in this gene may be associated with the neurological condition hyperplexia and also lead to molybdenum cofactor deficiency. Numerous alternatively spliced transcript variants encoding different isoforms have been described; however, the full-length nature of all transcript variants is not currently known.

Immunogen: E.coli-derived human Gephyrin/GPHN recombinant protein (Position: K148-L736).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Butler, M. H., Hayashi, A., Ohkoshi, N., Villmann, C., Becker, C.-M., Feng, G., De Camilli, P., Solimena, M. Autoimmunity to gephyrin in stiff-man syndrome. Neuron 26: 307-312, 2000. 2. Eguchi, M., Eguchi-Ishimae, M., Greaves, M. The small oligomerization domain of gephyrin converts MLL to an oncogene. Blood 103: 3876-3882, 2004. 3. Eguchi, M., Eguchi-Ishimae, M., Seto, M., Morishita, K., Suzuki, K., Ueda, R., Ueda, K., Kamada, N., Greaves, M. GPHN, a novel partner gene fused to MLL in a leukemia with t(11;14)(q23;q24). Genes Chromosomes Cancer 32: 212-221, 2001.

Uniprot ID: Q9NQX3

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 93 kDa

Calculated Molecular Weight:

Gene ID: 10243

Protein Name: Gephyrin [Includes: Molybdopterin adenylyltransferase

Gene Full Name: gephyrin

Synonyms: Domain E; GEPH; gephyrin; GPH; GPHN; GPHRYN; KIAA1385; MPT adenylyltransferase; MPT Mo transferase