Anti-GCNT2 Antibody Picoband®

SKU: A07194

Size: 100 μg

Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,ICC/IF,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml

Gene Name: GCNT2

Specificity:

Background: This gene encodes the enzyme responsible for formation of the blood group I antigen. The i and I antigens are distinguished by linear and branched poly-N-acetyllactosaminoglycans, respectively. The encoded protein is the I-branching enzyme, a beta-1,6-N-acetylglucosaminyltransferase responsible for the conversion of fetal i antigen to adult I antigen in erythrocytes during embryonic development. Mutations in this gene have been associated with adult i blood group phenotype. Alternatively spliced transcript variants encoding different isoforms have been described.

Immunogen: E.coli-derived human GCNT2 recombinant protein (Position: E27-Q397). Human GCNT2 shares 71.7% amino acid (aa) sequence identity with mouse GCNT2.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Bierhuizen, M. F. A., Mattei, M.-G., Fukuda, M. Expression of the developmental I antigen by a cloned human cDNA encoding a member of a beta-1,6-N-acetylglucosaminyltransferase gene family. Genes Dev. 7: 468-478, 1993. 2. Lin-Chu, M., Broadberry, R. E., Okubo, Y., Tanaka, M. The i phenotype and congenital cataracts among Chinese in Taiwan (Letter) Transfusion 31: 676-677, 1991. 3. Marsh, W. L., DePalma, H. Association between the Ii blood group and congenital cataract. Transfusion 22: 337-338, 1982.

Uniprot ID: Q8N0V5

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 46 kDa

Calculated Molecular Weight: 46 kDa

Gene ID: 2651

Protein Name: N-acetyllactosaminide beta-1,6-N-acetylglucosaminyl-transferase

Gene Full Name: glucosaminyl (N-acetyl) transferase 2 (I blood group)

Synonyms: IGNT; I-branching enzyme; GCNT5; GCNT2C; EC:2.4.1.150