Anti-GBP1 Antibody Picoband®

SKU: A03067-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: ELISA,Flow Cytometry,IP,IF,IHC,ICC,WB

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Immunoprecipitation, 0.5-2 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: GBP1

Specificity:

Background: Interferon-induced guanylate-binding protein 1 is a protein that in humans is encoded by the GBP1 gene. It belongs to the dynamin superfamily of large GTPases. Guanylate binding protein expression is induced by interferon. Guanylate binding proteins are characterized by their ability to specifically bind guanine nucleotides (GMP, GDP, and GTP) and are distinguished from the GTP-binding proteins by the presence of 2 binding motifs rather than 3.

Immunogen: E.coli-derived human GBP1 recombinant protein (Position: E190-S592).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Cheng, Y.-S. E., Patterson, C. E., Staeheli, P. Interferon-induced guanylate-binding proteins lack an N(T)KXD consensus motif and bind GMP in addition to GDP and GTP. Molec. Cell. Biol. 11: 4717-4725, 1991. 2. Gaudet, R. G., Zhu, S., Halder, A., Kim, B.-H., Bradfield, C. J., Huang, S., Xu, D., Maminska, A., Nguyen, T. N., Lazarou, M., Karatekin, E., Gupta, K., MacMicking, J. D. A human apolipoprotein L with detergent-like activity kills intracellular pathogens. Science 373: eabf8113, 2021. 3. Ghosh, A., Praefcke, G. J. K., Renault, L., Wittinghofer, A., Herrmann, C. How guanylate-binding proteins achieve assembly-stimulated processive cleavage of GTP to GMP. Nature 440: 101-104, 2006.

Uniprot ID: P32455

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity:

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 68 kDa

Calculated Molecular Weight: 68 kDa

Gene ID: 2633

Protein Name: Guanylate-binding protein 1

Gene Full Name: guanylate binding protein 1

Synonyms: GBP 1; GBP1; GTP binding protein 1; HuGBP 1