Anti-GBF1 Antibody Picoband®

SKU: A01817-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: GBF1

Specificity:

Background: Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 is a protein that in humans is encoded by the GBF1 gene. This gene encodes a member of the Sec7 domain family. The encoded protein is a guanine nucleotide exchange factor that regulates the recruitment of proteins to membranes by mediating GDP to GTP exchange. The encoded protein is localized to the Golgi apparatus and plays a role in vesicular trafficking by activating ADP ribosylation factor 1. The encoded protein has also been identified as an important host factor for viral replication. Multiple transcript variants have been observed for this gene.

Immunogen: E.coli-derived human GBF1 recombinant protein (Position: E110-K1600).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Mansour, S. J., Herbrick, J.-A., Scherer, S. W., Melancon, P. Human GBF1 is a ubiquitously expressed gene of the Sec7 domain family mapping to 10q24. Genomics 54: 323-327, 1998. 2. Mendoza-Ferreira, N., Karakaya, M., Cengiz, N., Beijer, D., Brigatti, K. W., Gonzaga-Jauregui, C., Fuhrmann, N., Holker, I., Thelen, M. P., Zetzsche, S., Rombo, R., Puffenberger, E. G., and 9 others. De novo and inherited variants in GBF1 are associated with axonal neuropathy caused by golgi fragmentation. Am. J. Hum. Genet. 107: 763-777, 2020. 3. Nagase, T., Seki, N., Ishikawa, K., Ohira, M., Kawarabayasi, Y., Ohara, O., Tanaka, A., Kotani, H., Miyajima, N., Nomura, N. Prediction of the coding sequences of unidentified human genes. VI. The coding sequences of 80 new genes (KIAA0201-KIAA0280) deduced by analysis of cDNA clones from cell line KG-1 and brain. DNA Res. 3: 321-329, 1996.

Uniprot ID: Q92538

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity:

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 250 kDa

Calculated Molecular Weight: 207 kDa

Gene ID: 8729

Protein Name: Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1

Gene Full Name: golgi brefeldin A resistant guanine nucleotide exchange factor 1

Synonyms: GBF1; KIAA0248; Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1; BFA-resistant GEF 1