Anti-FHOD1 Antibody Picoband®

SKU: A07228-1

Size: 100 μg

Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml

Gene Name: FHOD1

Specificity:

Background: This gene encodes a protein which is a member of the formin/diaphanous family of proteins. The gene is ubiquitously expressed but is found in abundance in the spleen. The encoded protein has sequence homology to diaphanous and formin proteins within the Formin Homology (FH)1 and FH2 domains. It also contains a coiled-coil domain, a collagen-like domain, two nuclear localization signals, and several potential PKC and PKA phosphorylation sites. It is a predominantly cytoplasmic protein and is expressed in a variety of human cell lines. Alternative splicing results in multiple transcript variants.

Immunogen: E.coli-derived human FHOD1 recombinant protein (Position: D290-R1121). Human FHOD1 shares 82.2% amino acid (aa) sequence identity with mouse FHOD1.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Sanematsu, F., Kanai, A., Ushijima, T., Shiraishi, A., Abe, T., Kage, Y., Sumimoto, H., Takeya, R. Fhod1, an actin-organizing formin family protein, is dispensable for cardiac development and function in mice. Cytoskeleton 76: 219-229, 2019. 2. Westendorf, J. J., Koka, S. Identification of FHOD1-binding proteins and mechanisms of FHOD1-regulated actin dynamics. J. Cell. Biochem. 92: 29-41, 2004. 3. Westendorf, J. J., Mernaugh, R., Hiebert, S. W. Identification and characterization of a protein containing formin homology (FH1/FH2) domains Gene 232: 173-182, 1999.

Uniprot ID: Q9Y613

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 140 kDa

Calculated Molecular Weight: 127 kDa

Gene ID: 29109

Protein Name: FH1/FH2 domain-containing protein 1

Gene Full Name: formin homology 2 domain containing 1

Synonyms: FHOD1; FHOS; FHOS1; FH1/FH2 domain-containing protein 1; Formin homolog overexpressed in spleen 1; FHOS; Formin homology 2 domain-containing protein 1