Anti-EB1/MAPRE1 Antibody Picoband®

SKU: A02070-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,Flow Cytometry,ELISA

Application Details: Western blot, 0.1-0.25 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: MAPRE1

Specificity:

Background: Microtubule-associated protein RP/EB family member 1 is a protein that in humans is encoded by the MAPRE1 gene. The protein encoded by this gene was first identified by its binding to the APC protein which is often mutated in familial and sporadic forms of colorectal cancer. This protein localizes to microtubules, especially the growing ends, in interphase cells. During mitosis, the protein is associated with the centrosomes and spindle microtubules. The protein also associates with components of the dynactin complex and the intermediate chain of cytoplasmic dynein. Because of these associations, it is thought that this protein is involved in the regulation of microtubule structures and chromosome stability. This gene is a member of the RP/EB family.

Immunogen: E.coli-derived human EB1/MAPRE1 recombinant protein (Position: A145-Y268).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Berrueta, L., Kraeft, S.-K., Tirnauer, J. S., Schuyler, S. C., Chen, L. B., Hill, D. E., Pellman, D., Bierer, B. E. The adenomatous polyposis coli-binding protein EB1 is associated with cytoplasmic and spindle microtubules. Proc. Nat. Acad. Sci. 95: 10596-10601, 1998. 2. Berrueta, L., Tirnauer, J. S., Schuyler, S. C., Pellman, D., Bierer, B. E. The APC-associated protein EB1 associates with components of the dynactin complex and cytoplasmic dynein intermediate chain. Curr. Biol. 9: 425-428, 1999. 3. Jiang, K., Wang, J., Liu, J., Ward, T., Wordeman, L., Davidson, A., Wang, F, Yao, X. TIP150 interacts with and targets MCAK at the microtubule plus ends. EMBO Rep. 10: 857-865, 2009.

Uniprot ID: Q15691

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 35 kDa

Calculated Molecular Weight:

Gene ID: 22919

Protein Name: Microtubule-associated protein RP/EB family member 1

Gene Full Name: microtubule associated protein RP/EB family member 1

Synonyms: APC binding protein EB1; EB1; End binding protein 1; MAPRE1