Anti-Dopamine Receptor D5/DRD5 Antibody Picoband®

SKU: PA2204

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Mouse,Rat

Applications: WB

Application Details: Western blot, 0.1-0.5μg/ml, Mouse, Rat

Gene Name: DRD5

Specificity: No cross reactivity with other proteins.

Background: D (1B) dopamine receptor (DRD5), also called DRD1B or DRD1L2 is a protein that in humans is encoded by the DRD5 gene. This gene is mapped to 4p16.1. This gene encodes the D5 subtype of the dopamine receptor. The D5 subtype is a G-protein coupled receptor which stimulates adenylyl cyclase. This receptor is expressed in neurons in the limbic regions of the brain. It has a 10-fold higher affinity for dopamine than the D1 subtype. Pseudogenes related to this gene reside on chromosomes 1 and 2. Compared with DRD1, DRD5 displayed a higher affinity for dopamine and was able to stimulate a biphasic rather than a monophasic intracellular accumulation of cAMP.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of mouse Dopamine Receptor D5, different from the related rat sequence by two amino acids, and from the related human sequence by three amino acids.

Clonality: Polyclonal

Contents: Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Li, H., Armando, I., Yu, P., Escano, C., Mueller, S. C., Asico, L., Pascua, A., Lu, Q., Wang, X., Villar, V. A. M., Jones, J. E., Wang, Z., and 9 others. Dopamine 5 receptor mediates Ang II type 1 receptor degradation via a ubiquitin-proteasome pathway in mice and human cells. J. Clin. Invest. 118: 2180-2189, 2008. Note: Erratum: J. Clin. Invest. 118: 2986 only, 2008. 2. Lowe, N., Kirley, A., Hawi, Z., Sham, P., Wickham, H., Kratochvil, C. J., Smith, S. D., Lee, S. Y., Levy, F., Kent, L., Middle, F., Rohde, L. A., and 20 others. Joint analysis of the DRD5 marker concludes association with attention-deficit/hyperactivity disorder confined to the predominantly inattentive and combined subtypes. Am. J. Hum. Genet. 74: 348-356, 2004. 3. Misbahuddin, A., Placzek, M. R., Chaudhuri, K. R., Wood, N. W., Bhatia, K. P., Warner, T. T. A polymorphism in the dopamine receptor DRD5 is associated with blepharospasm. Neurology 58: 124-126, 2002.

Uniprot ID: Q8BLD9

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 53 kDa

Calculated Molecular Weight: 53076 MW

Gene ID: 13492

Protein Name: D

Gene Full Name: dopamine receptor D5

Synonyms: D(1B) dopamine receptor; D(5) dopamine receptor; D1beta dopamine receptor; DBDR; Dopamine D5 receptor; dopamine receptor D5; DRD1B; DRD1L2; DRD5