Anti-DNA Polymerase iota/POLI Antibody Picoband®

SKU: A01376-1

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB

Application Details: Western blot, 0.1-0.5μg/ml

Gene Name: POLI

Specificity: No cross reactivity with other proteins.

Background: DNA polymerase iota is an enzyme that in humans is encoded by the POLI gene. The protein encoded by this gene is an error-prone DNA polymerase involved in DNA repair. The encoded protein promotes DNA synthesis across lesions in the template DNA, which other polymerases cannot do. The encoded polymerase inserts deoxynucleotides across lesions and then relies on DNA polymerase zeta to extend the nascent DNA strand to bypass the lesion.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human DNA Polymerase iota, which shares 70.6% and 76.5% amino acid (aa) sequence identity with mouse and rat DNA Polymerase iota, respectively.

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Frank EG, Woodgate R (Aug 2007). Increased catalytic activity and altered fidelity of human DNA polymerase iota in the presence of manganese. J Biol Chem. 282 (34): 24689–96. 2. McDonald JP, Tissier A, Frank EG, et al. (2001). DNA polymerase iota and related rad30-like enzymes.. Philos. Trans. R. Soc. Lond., B, Biol. Sci. 356 (1405): 53–60.

Uniprot ID: Q9UNA4

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 83 kDa

Calculated Molecular Weight:

Gene ID: 11201

Protein Name: DNA polymerase iota

Gene Full Name: DNA polymerase iota

Synonyms: DNA polymerase iota; Eta2; POLI; polymerase (DNA directed) iota; RAD30 homolog B; RAD30B; RAD3OB