Anti-DIO1 Antibody Picoband®

SKU: A04612

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: ELISA,Flow Cytometry,IF,ICC,WB

Application Details: Western blot, 0.25-0.5μg/ml, Human, Rat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human, Mouse
ELISA, 0.1-0.5μg/ml, -

Gene Name: DIO1

Specificity: No cross reactivity with other proteins.

Background: Type I iodothyronine deiodinase is a protein that in humans is encoded by the DIO1 gene. It is mapped to 1p32.3. The protein encoded by this gene belongs to the iodothyronine deiodinase family. It catalyzes the activation, as well as the inactivation of thyroid hormone by outer and inner ring deiodination, respectively. The activation reaction involves the conversion of the prohormone thyroxine (3,5,3',5'-tetraiodothyronine, T4), secreted by the thyroid gland, to the bioactive thyroid hormone (3,5,3'-triiodothyronine, T3) by 5'-deiodination. This protein provides most of the circulating T3, which is essential for growth, differentiation and basal metabolism in vertebrates. This protein is a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Alternatively spliced transcript variants have been found for this gene.

Immunogen: E.coli-derived human DIO1 recombinant protein (Position: K137-S249).

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Berry, M. J., Banu, L., Larsen, P. R. Type I iodothyronine deiodinase is a selenocysteine-containing enzyme. Nature 349: 438-440, 1991. 2. Berry, M. J., Grieco, D., Taylor, B. A., Maia, A. L., Kieffer, J. D., Beamer, W., Glover, E., Poland, A., Larsen, P. R. Physiological and genetic analyses of inbred mouse strains with a type I iodothyronine 5-prime deiodinase deficiency. J. Clin. Invest. 92: 1517-1528, 1993. 3. de Jong, F. J., Peeters, R. P., den Heijer, T., van der Deure, W. M., Hofman, A., Uitterlinden, A. G., Visser, T. J., Breteler, M. M. B. The association of polymorphisms in the type 1 and 2 deiodinase genes with circulating thyroid hormone parameters and atrophy of the medial temporal lobe. J. Clin. Endocr. Metab. 92: 636-640, 2007.

Uniprot ID: P49895

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 29 kDa

Calculated Molecular Weight:

Gene ID: 1733

Protein Name: Type I iodothyronine deiodinase

Gene Full Name: iodothyronine deiodinase 1

Synonyms: 5DI; DIO1; DIOI; ITDI1; TXDI1; Type 1 DI; Type I 5 deiodinase