Anti-DIAPH1 Antibody Picoband®

SKU: A02308-3

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Monkey

Applications: ELISA,Flow Cytometry,IF,ICC,WB

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Monkey
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: DIAPH1

Specificity:

Background: Protein diaphanous homolog 1 is a protein that in humans is encoded by the DIAPH1 gene. This gene is a homolog of the Drosophila diaphanous gene, and has been linked to autosomal dominant, fully penetrant, nonsyndromic sensorineural progressive low-frequency hearing loss. Actin polymerization involves proteins known to interact with diaphanous protein in Drosophila and mouse. It has therefore been speculated that this gene may have a role in the regulation of actin polymerization in hair cells of the inner ear. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Immunogen: E.coli-derived human DIAPH1 recombinant protein (Position: D1039-Q1153).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Al-Maawali, A., Barry, B. J., Rajab, A., El-Quessny, M., Seman, A., Coury, S. N., Barkovich, A. J., Yang, E., Walsh, C. A., Mochida, G. H., Stoler, J. M. Novel loss-of-function variants in DIAPH1 associated with syndromic microcephaly, blindness, and early onset seizures. Am. J. Med. Genet. 170A: 435-440, 2016. 2. Carreira, S., Goodall, J., Denat, L., Rodriguez, M., Nuciforo, P., Hoek, K. S., Testori, A., Larue, L., Goding, C. R. Mitf regulation of Dia1 controls melanoma proliferation and invasiveness. Genes Dev. 20: 3426-3439, 2006. 3. Ercan-Sencicek, A. G., Jambi, S., Franjic, D., Nishimura, S., Li, M., El-Fishawy, P., Morgan, T. M., Sanders, S. J., Bilguvar, K., Suri, M., Johnson, M. H., Gupta, A. R., Yuksel, Z., Mane, S., Grigorenko, E., Picciotto, M., Alberts, A. S., Gunel, M., Sestan, N., State, M. W. Homozygous loss of DIAPH1 is a novel cause of microcephaly in humans. Europ. J. Hum. Genet. 23: 165-172, 2015.

Uniprot ID: O60610

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 150 kDa

Calculated Molecular Weight: 33927 MW

Gene ID: 1729

Protein Name: Protein diaphanous homolog 1

Gene Full Name: diaphanous related formin 1

Synonyms: DFNA1; DIA1; DIAP1; DIAPH1; Diaphanous related formin 1; hDIA1; mDia1; Protein diaphanous homolog 1