Anti-DAX-1/NR0B1 Antibody Picoband®

SKU: A01521-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: ELISA,IF,ICC,WB

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: NR0B1

Specificity:

Background: DAX1 (dosage-sensitive sex reversal, adrenal hypoplasia critical region, on chromosome X, gene 1) is a nuclear receptor protein that in humans is encoded by the NR0B1 gene (nuclear receptor subfamily 0, group B, member 1). This gene encodes a protein that contains a DNA-binding domain. The encoded protein acts as a dominant-negative regulator of transcription which is mediated by the retinoic acid receptor. This protein also functions as an anti-testis gene by acting antagonistically to Sry. Mutations in this gene result in both X-linked congenital adrenal hypoplasia and hypogonadotropic hypogonadism.

Immunogen: E.coli-derived human DAX-1/NR0B1 recombinant protein (Position: V126-Q404).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Achermann, J. C., Gu, W.-X., Kotlar, T. J., Meeks, J. J., Sabacan, L. P., Seminara, S. B., Habiby, R. L., Hindmarsh, P. C., Bick, D. P., Sherins, R. J., Crowley, W. F., Jr., Layman, L. C., Jameson, J. L. Mutational analysis of DAX1 in patients with hypogonadotropic hypogonadism or pubertal delay. J. Clin. Endocr. Metab. 84: 4497-4500, 1999. 2. Achermann, J. C., Ito, M., Ito, M., Hindmarsh, P. C., Jameson, J. L. A mutation in the gene encoding steroidogenic factor-1 causes XY sex reversal and adrenal failure in humans. (Letter) Nature Genet. 22: 125-126, 1999. 3. Achermann, J. C., Silverman, B. L., Habiby, R. L., Jameson, J. L. Presymptomatic diagnosis of X-linked adrenal hypoplasia congenita by analysis of DAX1. J. Pediat. 137: 878-881, 2000.

Uniprot ID: P51843

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 48 kDa

Calculated Molecular Weight:

Gene ID: 190

Protein Name: Nuclear receptor subfamily 0 group B member 1

Gene Full Name: nuclear receptor subfamily 0 group B member 1

Synonyms: AHC; AHCH; AHX; DAX 1; DAX1; DSS; GTD; HHG; NR0B1; NROB1; Nuclear receptor DAX 1