Anti-Cytokeratin 19/KRT19 Antibody Picoband®

SKU: PA1335

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human

Applications: IHC,ICC,WB

Application Details: Immunocytochemistry , 0.5-1μg/ml, Human, -
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human, -
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
Western blot, 0.1-0.5μg/ml, Human

Gene Name: KRT19

Specificity: No cross reactivity with other proteins.

Background: Keratin, type I cytoskeletal 19 is a protein that in humans is encoded by the KRT19 gene.The protein encoded by this gene is a member of the keratin family. It is specifically expressed in the periderm, the transiently superficial layer that envelops the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21. Due to its high sensitivity, KRT19 is the most used marker for the RT-PCR-mediated detection of tumor cells disseminated in lymph nodes, peripheral blood, and bone marrow of breast cancer patients.Keratin 19 is often used together with keratin 8 and keratin 18 to differentiate cells of epithelial origin from hematopoietic cells in tests that enumerate circulating tumor cells in blood.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Cytokeratin 19.

Clonality: Polyclonal

Contents: Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Schweizer J, Bowden PE, Coulombe PA, Langbein L, Lane EB, Magin TM, Maltais L, Omary MB, Parry DA, Rogers MA, Wright MW (Jul 2006). New consensus nomenclature for mammalian keratins. J Cell Biol 174 (2): 169–74. 2. Entrez Gene: KRT19 keratin 19. 3. W. Jeffrey Allard, Jeri Matera, M. Craig Miller, et al. (October 2004). Tumor Cells Circulate in the Peripheral Blood of All Major Carcinomas but not in Healthy Subjects or Patients With Nonmalignant Diseases. Clin. Cancer Research 10 (20): 6897–6904.

Uniprot ID: P08727

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 44 kDa

Calculated Molecular Weight: 44106 MW

Gene ID: 3880

Protein Name: Keratin, type I cytoskeletal 19

Gene Full Name: keratin 19

Synonyms: Cell and organelle markers; CK 19; CK19; Cytokeratin 19; Cytoskeleton Marker; K19; K1CS; keratin 19; KRT19