Anti-Cytochrome P450 17A1/Cyp17a1 Antibody Picoband®

SKU: A00615-3

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Mouse,Rat

Applications: ELISA,IF,IHC,WB

Application Details: Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Immunofluorescence, 2μg/ml
ELISA, 0.1-0.5μg/ml

Gene Name: Cyp17a1

Specificity: No cross reactivity with other proteins.

Background: Cytochrome P450 17A1, also called steroid 17α-monooxygenase, is an enzyme of the hydroxylase type that in humans is encoded by the CYP17A1 gene on chromosome 10. This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum. It has both 17alpha-hydroxylase and 17,20-lyase activities and is a key enzyme in the steroidogenic pathway that produces progestins, mineralocorticoids, glucocorticoids, androgens, and estrogens. Mutations in this gene are associated with isolated steroid-17 alpha-hydroxylase deficiency, 17-alpha-hydroxylase/17,20-lyase deficiency, pseudohermaphroditism, and adrenal hyperplasia.

Immunogen: E. coli-derived mouse Cyp17a1 recombinant protein (Position: Q80-R363).

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. DeVore NM, Scott EE (February 2012). Structures of cytochrome P450 17A1 with prostate cancer drugs abiraterone and TOK-001. Nature. 482(7383): 116–9. 2. Estrada DF, Laurence JS, Scott EE (February 2016). Cytochrome P450 17A1 Interactions with the FMN Domain of Its Reductase as Characterized by NMR. The Journal of Biological Chemistry. 291 (8): 3990–4003. 3. Petrunak EM, DeVore NM, Porubsky PR, Scott EE (November 2014). Structures of human steroidogenic cytochrome P450 17A1 with substrates.The Journal of Biological Chemistry. 289 (47): 32952–64.

Uniprot ID: P27786

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 57 kDa

Calculated Molecular Weight: 57371 MW

Gene ID: 13074

Protein Name: Steroid 17-alpha-hydroxylase/17,20 lyase

Gene Full Name: cytochrome P450, family 17, subfamily a, polypeptide 1

Synonyms: CPT7; CYP17; CYP17A1; CYPXVII; Cytochrome P450 17A1; Cytochrome P450 C17; Cytochrome P450c17; P450C17; S17AH; Steroid 17 alpha monooxygenase