Anti-Collagen, Type III Col3a1 Antibody (Monoclonal, FH-7A)

SKU: MA1029

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Rat

Applications: IHC,WB

Application Details: Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human, rat, -
Western blot, 0.25-0.5μg/ml, Human, rat

Gene Name: COL3A1

Specificity: No cross reactivity with other proteins.

Background: Collagen type III, also known as COL3A1, is assigned to chromosome 2. Ultrastructural analysis of tissues from mutant mice revealed that type III collagen is essential for normal collagen I fibrillogenesis in the cardiovascular system and other organs. Type III collagen is crucial for collagen I fibrillogenesis and for normal cardiovascular development.

Immunogen: Human collagen type III.

Clonality: Monoclonal

Contents: Mouse IgG in stabilizing components, 1.2% sodium acetate and 0.01mg NaN3. *This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Purification: Ascites

Reconstitution: Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Reference: 1. Cutting, G. R.; McGinniss, M. J.; Kasch, I. M.; Tsipouras, P.; Antonarakis, S. E. : Physical mapping by PFGE localizes the COL3A1 and COL5A2 genes to a 35 kb region on chromosome 2. (Abstract) Clin. Res. 38: 266A, 1990. 2. Emanuel, B. S.; Cannizzaro, L. A.; Seyer, J. M.; Myers, J. C. : Human alpha-1(III) and alpha-2(V) procollagen genes are located on the long arm of chromosome 2. Proc. Nat. Acad. Sci. 82: 3385-3389, 1985. 3. Liu, X.; Wu, H.; Byrne, M.; Krane, S.; Jaenisch, R. : Type III collagen is crucial for collagen I fibrillogenesis and for normal cardiovascular development. Proc. Nat. Acad. Sci. 94: 1852-1856, 1997.

Uniprot ID: P13941

Host: Mouse

Concentration: Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Mouse IgG1

Phospho_site:

Clone Number: Clone: FH-7A

Observed Molecular Weight: 49 kDa

Calculated Molecular Weight: 138936 MW

Gene ID: Human(1281), Mouse(12825), Rat(84032)

Protein Name: Collagen alpha-1

Gene Full Name: collagen type III alpha 1 chain

Synonyms: EDS4A; COLLAGEN, FETAL; COLLAGEN, BLOOD VESSEL