Anti-COBRA1/NELFB Antibody Picoband®

SKU: A12025-1

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: ELISA,WB

Application Details: Western blot, 0.1-0.5μg/ml
ELISA, 0.1-0.5μg/ml

Gene Name: NELFB

Specificity: No cross reactivity with other proteins.

Background: Cofactor of BRCA1, also known as COBRA1, is a human gene that encodes NELF-B. NELF-B is a subunit of negative elongation factor (NELF), which also includes NELF-A (WHSC2), either NELF-C or NELF-D (TH1L), and NELF-E (RDBP). NELF acts with DRB sensitivity-inducing factor (DSIF), a heterodimer of SPT4 (SUPT4H1) and SPT5 (SUPT5H), to cause transcriptional pausing of RNA polymerase II. COBRA1 was initially identified in a yeast two-hybrid screen using the BRCT1 domain of BRCA1 as bait.

Immunogen: E. coli-derived human COBRA1 recombinant protein (Position: M1-E252).

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Narita T, Yamaguchi Y, Yano K, Sugimoto S, Chanarat S, Wada T, Kim DK, Hasegawa J, Omori M, Inukai N, Endoh M, Yamada T, Handa H (2003). Human Transcription Elongation Factor NELF: Identification of Novel Subunits and Reconstitution of the Functionally Active Complex. Molecular and Cellular Biology. 23 (6): 1863–1873. 2. Ye Q, Hu YF, Zhong H, Nye AC, Belmont AS, Li R (2001). BRCA1-induced large-scale chromatin unfolding and allele-specific effects of cancer-predisposing mutations. The Journal of Cell Biology. 155 (6): 911–922.

Uniprot ID: Q8WX92

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 66 kDa

Calculated Molecular Weight:

Gene ID: 25920

Protein Name: Negative elongation factor B

Gene Full Name: negative elongation factor complex member B

Synonyms: COBRA1; cofactor of BRCA1; KIAA1182; Negative elongation factor B; NELF B; NELFB