Anti-CISD2 Antibody Picoband®

SKU: A06387-1

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Monkey,Mouse,Rat

Applications: WB,IHC,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat, Monkey
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Rat
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human
ELISA, 0.1-0.5μg/ml, -

Gene Name: CISD2

Specificity:

Background: CDGSH iron sulfur domain 2 is a protein that in humans is encoded by the CISD2 gene. The protein encoded by this gene is a zinc finger protein that localizes to the endoplasmic reticulum. The encoded protein binds an iron/sulfur cluster and may be involved in calcium homeostasis. Defects in this gene are a cause of Wolfram syndrome 2.

Immunogen: E.coli-derived human CISD2 recombinant protein (Position: A55-N124).

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Amr, S., Heisey, C., Zhang, M., Xia, X.-J., Shows, K. H., Ajlouni, K., Pandya, A., Satin, L. S., El-Shanti, H., Shiang, R. A homozygous mutation in a novel zinc-finger protein, ERIS, is responsible for Wolfram syndrome 2. Am. J. Hum. Genet. 81: 673-683, 2007. 2. Cattaneo, M., La Sala, L., Rondinelli, M., Errichiello, E., Zuffardi, O., Puca, A. A., Genovese, S., Ceriello, A. A donor splice site mutation in CISD2 generates multiple truncated, non-functional isoforms in Wolfram syndrome type 2 patients. BMC Med. Genet. 18: 147, 2017. 3. Chang, N. C., Nguyen, M., Bourdon, J., Risse, P.-A., Martin, J., Danialou, G., Rizzuto, R., Petrof, B. J., Shore, G. C. Bcl-2-associated autophagy regulator Naf-1 required for maintenance of skeletal muscle. Hum. Molec. Genet. 21: 2277-2287, 2012.

Uniprot ID: Q8N5K1

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 15 kDa

Calculated Molecular Weight: 26056 MW

Gene ID: 493856

Protein Name: CDGSH iron-sulfur domain-containing protein 2

Gene Full Name: CDGSH iron sulfur domain 2

Synonyms: CDGSH iron sulfur domain 2; CDGSH2; CISD2; ERIS; Miner1; MitoNEET related 1 protein; NAF 1; WFS2; ZCD2