Anti-Chromogranin A/CGA Antibody Picoband®

SKU: A00739-1

Size: 100 μg

Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,Flow Cytometry

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Gene Name: CGA

Specificity:

Background: The four human glycoprotein hormones chorionic gonadotropin (CG), luteinizing hormone (LH), follicle stimulating hormone (FSH), and thyroid stimulating hormone (TSH) are dimers consisting of alpha and beta subunits that are associated noncovalently. The alpha subunits of these hormones are identical, however, their beta chains are unique and confer biological specificity. The protein encoded by this gene is the alpha subunit and belongs to the glycoprotein hormones alpha chain family. Two transcript variants encoding different isoforms have been found for this gene.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Chromogranin A/CGA, which shares 78.3% amino acid (aa) sequence identity with mouse Chromogranin A/CGA.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Boothby, M., Ruddon, R. W., Anderson, C., McWilliams, D., Boime, I. A single gonadotropin alpha-subunit gene in normal tissue and tumor-derived cell lines. J. Biol. Chem. 256: 5121-5127, 1981. 2. Bordelon, M., Kohler, P. O. Synthesis of human glycoprotein hormone in somatic cell hybrids. (Abstract) J. Cell Biol. 67: 37A only, 1975. 3. Bordelon-Riser, M. E., Siciliano, M. J., Kohler, P. O. Necessity for two human chromosomes for human chorionic gonadotropin production in human-mouse hybrids. Somat. Cell Genet. 5: 597-613, 1979.

Uniprot ID: P01215

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 17 kDa

Calculated Molecular Weight: 13 kDa

Gene ID: 1081

Protein Name: Glycoprotein hormones alpha chain

Gene Full Name: glycoprotein hormones, alpha polypeptide

Synonyms: CGA; HCG; LHA; FSHA; GPA1; GPHa; TSHA; GPHA1; CG-ALPHA