Anti-CDC7 Antibody Picoband®

SKU: A01190-3

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: CDC7

Specificity:

Background: Cell division cycle 7-related protein kinase is an enzyme that in humans is encoded by the CDC7 gene. This gene encodes a cell division cycle protein with kinase activity that is critical for the G1/S transition. The yeast homolog is also essential for initiation of DNA replication as cell division occurs. Overexpression of this gene product may be associated with neoplastic transformation for some tumors. Multiple alternatively spliced transcript variants that encode the same protein have been detected.

Immunogen: E.coli-derived human CDC7 recombinant protein (Position: Q152-L574).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Hess, G. F., Drong, R. F., Weiland, K. L., Slightom, J. L., Sclafani, R. A., Hollingsworth, R. E. A human homolog of the yeast CDC7 gene is overexpressed in some tumors and transformed cell lines. Gene 211: 133-140, 1998. 2. Jiang, W., Hunter, T. Identification and characterization of a human protein kinase related to budding yeast Cdc7p. Proc. Nat. Acad. Sci. 94: 14320-14325, 1997. 3. Kim, J. M., Sato, N., Yamada, M., Arai, K., Masai, H. Growth regulation of the expression of mouse cDNA and gene encoding a serine/threonine kinase related to Saccharomyces cerevisiae CDC7 essential for G(1)/S transition: structure, chromosomal localization, and expression of mouse gene for S. cerevisiae CDC7-related kinase. J. Biol. Chem. 273: 23248-23257, 1998. Note: Erratum: J. Biol. Chem. 273: 27755 only, 1998.

Uniprot ID: O00311

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 64 kDa

Calculated Molecular Weight:

Gene ID: 8317

Protein Name: Cell division cycle 7-related protein kinase

Gene Full Name: cell division cycle 7

Synonyms: CDC7; CDC7 related kinase; CDC7L1; HsCDC7; Hsk1; huCDC7