Anti-CD9 Antibody Picoband®

SKU: A01202-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: ELISA,Flow Cytometry,IF,IHC,WB

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: CD9

Specificity:

Background: CD9 antigen is a protein that in humans is encoded by the CD9 gene. CD9 is a cell surface glycoprotein that is known to complex with integrins and other transmembrane 4 superfamily proteins. It is found on the surface of exosomes. It can modulate cell adhesion and migration and also trigger platelet activation and aggregation. In addition, the protein appears to promote muscle cell fusion and support myotube maintenance. This protein also seems to be a key part in the egg-sperm fusion during mammalian fertilization. While oocytes are ovulated, CD9-deficient oocytes are not properly fused with sperm upon fertilization. CD9 is located in the microvillar membrane of the oocytes and also appears to intervene in maintaining the normal shape of oocyte microvilli.

Immunogen: E.coli-derived human CD9 recombinant protein (Position: Q139-K192).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Katz F, Povey S, Parkar M, Schneider C, Sutherland R, Stanley K, Solomon E, Greaves M (March 1984). Chromosome assignment of monoclonal antibody-defined determinants on human leukemic cells. Eur J Immunol 13(12): 1008–1013. 2. Le Naour F, Rubinstein E, Jasmin C, Prenant M, Boucheix C (2000). Severely Reduced Female Fertility in CD9-Deficient Mice. Science 287(5451): 319–321. 3. Runge KE, Evans JE, He ZY, Gupta S, McDonald KL, Stahlberg H, Primakoff P, Myles DG (2007). Oocyte CD9 is enriched on the microvillar membrane and required for normal microvillar shape and distribution.Developmental Biology 304 (1): 317–325.

Uniprot ID: P21926

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 22 kDa

Calculated Molecular Weight:

Gene ID: 928

Protein Name: CD9 antigen

Gene Full Name: CD9 molecule

Synonyms: 5H9; 5H9 antigen; BA2; BTCC 1; CD9; CD9 antigen; CD9 molecule; Cell and organelle markers; DRAP 27; Exosome marker; GIG2; Leukocyte antigen MIC3; MIC3; Motility related protein; MRP 1; P24; Tetraspanin 29; Tspan 29; TSPAN29