Anti-CD23/FCER2 Antibody Picoband®

SKU: PB9051

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: ELISA (Cap),Flow Cytometry,IF,IHC,IHC-F,ICC,WB

Application Details: Western blot, 0.1-0.5μg/ml, Mouse, Rat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Mouse
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat
Immunocytochemistry, 0.5-1μg/ml, Human
Immunofluorescence, 2μg/ml, Mouse, Rat
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Mouse
ELISA (Cap), 1-5μg/ml, Mouse

Gene Name: FCER2

Specificity: No cross reactivity with other proteins.

Background: CD23, also known as Fc epsilon RII, or FcεRII, is the low-affinity receptor for IgE, an antibody isotype involved in allergy and resistance to parasites, and is important in regulation of IgE levels. There are two forms of CD23: CD23a and CD23b. CD23a is present on follicular B cells, whereas CD23b requires IL-4 to be expressed on T-cells, monocytes, Langerhans cells, eosinophils, and macrophages. As part of a mapping of multiple probes to specific bands on chromosome 19 by fluorescence in situ hybridization, the FCE2 gene was assigned to 19p13.3. CD23 (FCE2) is a key molecule for B-cell activation and growth. It is the low-affinity receptor for IgE. The truncated molecule can be secreted, then functioning as a potent mitogenic growth factor.

Immunogen: E.coli-derived mouse CD23 recombinant protein (Position: E50-P331). Mouse CD23 shares 52% amino acid (aa) sequence identity with human CD23.

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Lichtman AH, Abbas AK (2003). Cellular and molecular immunology. Philadelphia: Saunders. pp. 324–325. 2. Weskamp, G., Ford, J. W., Sturgill, J., Martin, S., Docherty, A. J. P., Swendeman, S., Broadway, N., Hartmann, D., Saftig, P., Umland, S., Sehara-Fujisawa, A., Black, R. A., Ludwig, A., Becherer, J. D., Conrad, D. H., Blobel, C. P.ADAM10 is a principal 'sheddase' of the low-affinity immunoglobulin E receptor CD23.Nature Immun. 7: 1293-1298, 2006.

Uniprot ID: P20693

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 49 kDa

Calculated Molecular Weight: 37648 MW

Gene ID: 14128

Protein Name: Low affinity immunoglobulin epsilon Fc receptor

Gene Full Name: Fc receptor, IgE, low affinity II, alpha polypeptide

Synonyms: BLAST 2; CD23; CD23A; CLEC4J; Fc epsilon RII; FCE2; FCER2; IGEBF; Lymphocyte IgE receptor