Anti-Ccl21 Antibody Picoband®

SKU: A00765-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Mouse,Rat

Applications: WB,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Rat
ELISA, 0.1-0.5 μg/ml, Rat

Gene Name: Ccl21

Specificity:

Background: Chemokine (C-C motif) ligand 21 (CCL21) is a small cytokine belonging to the CC chemokine family. This antimicrobial gene is one of several CC cytokine genes clustered on the p-arm of chromosome 9. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. Similar to other chemokines the protein encoded by this gene inhibits hemopoiesis and stimulates chemotaxis. This protein is chemotactic in vitro for thymocytes and activated T cells, but not for B cells, macrophages, or neutrophils. The cytokine encoded by this gene may also play a role in mediating homing of lymphocytes to secondary lymphoid organs. It is a high affinity functional ligand for chemokine receptor 7 that is expressed on T and B lymphocytes and a known receptor for another member of the cytokine family (small inducible cytokine A19).

Immunogen: E.coli-derived rat Ccl21 recombinant protein (Position: S24-Q133).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Dijkstra, I. M., Hulshof, S., van der Valk, P., Boddeke, H. W. G. M., Biber, K. Cutting edge: activity of human adult microglia in response to CC chemokine ligand 21. J. Immun. 172: 2744-2747, 2004. 2. Harhausen, D., Prinz, V., Ziegler, G., Gertz, K., Endres, M., Lehrach, H., Gasque, P., Botto, M., Stahel, P. F., Dirnagl, U., Nietfeld, W., Trendelenburg, G. CD93/AA4.1: a novel regulator of inflammation in murine focal cerebral ischemia. J. Immun. 184: 6407-6417, 2010. 3. Hedrick, J. A., Zlotnik, A. Identification and characterization of a novel beta chemokine containing six conserved cysteines. J. Immun. 159: 1589-1593, 1997.

Uniprot ID: Q5RJN3

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 15 kDa

Calculated Molecular Weight: 59195 MW

Gene ID: 298006

Protein Name: 6Ckine

Gene Full Name: C-C motif chemokine ligand 21

Synonyms: C-C motif chemokine ligand 21; 6Ckine; CKb9; ECL; SCYA21; SLC; TCA4; CCL21