Anti-Carbonic Anhydrase III/CA3 Antibody Picoband®

SKU: PA1439

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: IHC,WB

Application Details: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat

Gene Name: CA3

Specificity: No cross reactivity with other proteins.

Background: Carbonic anhydrase III (CA3) is an enzyme that in humans is encoded by the CA3 gene. CA3 is a member of a multigene family (at least six separate genes are known) that encode carbonic anhydrase isozymes. The gene spans 10.3 kb and contains seven exons and six introns. Using a cDNA clone of the CA3 gene in the study of human-rodent hybrids, the gene was mapped to chromosome 8 which carries a cluster of CA genes. CA3 is found in high concentration in muscle. CA3 is used in conjunction with creatine kinase detection of the carrier state for Duchenne muscular dystrophy.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human CA3, different from the related mouse and rat sequences by two amino acids.

Clonality: Polyclonal

Contents: Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Edwards, Y. H., Lloyd, J. C., Parkar, M., Povey, S.The gene for human muscle specific carbonic anhydrase (CAIII) is assigned to chromosome 8.Ann. Hum. Genet. 50: 41-47, 1986. 2. Heath, R., Carter, N. D., Jeffery, S., Edwards, R. J., Watts, D. C., Watts, R. L.Evaluation of carrier detection of Duchenne muscular dystrophy using carbonic anhydrase III and creatine kinase.Am. J. Med. Genet. 21: 291-296, 1985. 3. Wade, R., Gunning, P., Eddy, R., Shows, T., Kedes, L.Nucleotide sequence, tissue-specific expression, and chromosome location of human carbonic anhydrase III: the human CAIII gene is located on the same chromosome as the closely linked CAI and CAII genes.Proc. Nat. Acad. Sci. 83: 9571-9575, 1986.

Uniprot ID: P07451

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 30 kDa

Calculated Molecular Weight: 30 kDa

Gene ID: 761

Protein Name: Carbonic anhydrase 3

Gene Full Name: carbonic anhydrase 3

Synonyms: CA III; CA3; CAIII; Car3; Carbonate dehydratase III; Carbonic anhydrase 3; Carbonic anhydrase III