Anti-CaMKK/CAMKK1 Antibody Picoband®

SKU: PA1520

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: IHC,WB

Application Details: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Rat, Mouse
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Gene Name: CAMKK1

Specificity: No cross reactivity with other proteins.

Background: CAMKK1, Calcium/calmodulin-dependent protein kinase kinase 1 is an enzyme that in humans is encoded by the CAMKK1 gene. The CAMKK1 gene is mapped to chromosome 17. The product of this gene belongs to the Serine/Threonine protein kinase family, and to the Ca (2+)/calmodulin-dependent protein kinase subfamily. This protein plays a role in the calcium/calmodulin-dependent (CaM) kinase cascade. Three transcript variants encoding two distinct isoforms have been identified for this gene.Camkk1binds calmodulin and activated Camk4 with a 6-fold increase in total activity and a 100-fold increase in Camk4 Ca (2+)-independent activity. Camkk1 catalyzed a 10-fold increase in the total activity of Camk1 and had no effect on Camk2. Cotransfection of COS-7 cells with Camkk1 and Camk4 resulted in a 14-fold increase in CRE-binding protein-dependent transcription compared with Camk4 alone, suggesting that Camkk1 enhances Camk4-mediated transcriptional regulation.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of mouse CaMKK, different from the related human sequence by three amino acids, and from the related rat sequence by two amino acids.

Clonality: Polyclonal

Contents: Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Blaeser, F., Sanders, M. J., Truong, N., Ko, S., Wu, L. J., Wozniak, D. F., Fanselow, M. S., Zhuo, M., Chatila, T. A.Long-term memory deficits in pavlovian fear conditioning in Ca(2+)/calmodulin kinase kinase alpha-deficient mice.Molec. Cell. Biol. 26: 9105-9115, 2006. 2. Tokumitsu, H., Enslen, H., Soderling, T. R.Characterization of a Ca(2+)/calmodulin-dependent protein kinase cascade: molecular cloning and expression of calcium/calmodulin-dependent protein kinase kinase.J. Biol. Chem. 270: 19320-19324, 1995.

Uniprot ID: Q8VBY2

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 56 kDa

Calculated Molecular Weight: 56 kDa

Gene ID: 55984

Protein Name: Calcium/calmodulin-dependent protein kinase kinase 1

Gene Full Name: calcium/calmodulin-dependent protein kinase kinase 1, alpha

Synonyms: CaM kinase IV kinase; CaM kinase kinase 1; CaM kinase kinase alpha; CaM KK 1; CaM KK alpha; CaMKK 1; CaMKK alpha; CAMKK1; CAMKKA