Anti-BOP1 Antibody Picoband®

SKU: A04026-2

Size: 100 μg

Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,ICC/IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml

Gene Name: BOP1

Specificity:

Background: Enables RNA binding activity. Involved in regulation of cell cycle; regulation of signal transduction by p53 class mediator; and ribosomal large subunit biogenesis. Located in chromosome; nucleolus; and nucleoplasm. Part of PeBoW complex.

Immunogen: E.coli-derived human BOP1 recombinant protein (Position: R157-N701). Human BOP1 shares 89.9% and 89.7% amino acid (aa) sequence identity with mouse and rat BOP1, respectively.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Chen, B., Dragomir, M. P., Fabris, L., Bayraktar, R., Knutsen, E., Liu, X., Tang, C., Li, Y., Shimura, T., Ivkovic, T. C., Cruz de los Santos, M., Anfossi, S., and 36 others. The long noncoding RNA CCAT2 induces chromosomal instability through BOP1-AURKB signaling. Gastroenterology 159: 2146-2195, 2020. 2. Kellner, M., Rohrmoser, M., Forne, I., Voss, K., Burger, K., Muhl, B., Gruber-Eber, A., Kremmer, E., Imhof, A., Eick, D. DEAD-box helicase DDX27 regulates 3-prime end formation of ribosomal 47S RNA and stably associates with the PeBoW-complex. Exp. Cell Res. 334: 146-159, 2015. 3. Killian, A., Le Meur, N., Sesboue, R., Bourguignon, J., Bougeard, G., Gautherot, J., Bastard, C., Frebourg, T., Flaman, J.-M. Inactivation of the RRB1-pescadillo pathway involved in ribosome biogenesis induces chromosomal instability. Oncogene 23: 8597-8602, 2004.

Uniprot ID: Q14137

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 110 kDa

Calculated Molecular Weight: 84 kDa

Gene ID: 23246

Protein Name: Ribosome biogenesis protein BOP1

Gene Full Name: BOP1 ribosomal biogenesis factor

Synonyms: block of proliferation 1; BOP1; KIAA0124