Anti-BCCIP Antibody Picoband®

SKU: A05912-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: BCCIP

Specificity:

Background: BRCA2 and CDKN1A-interacting protein is a protein that in humans is encoded by the BCCIP gene. This gene product was isolated on the basis of its interaction with BRCA2 and p21 proteins. It is an evolutionarily conserved nuclear protein with multiple interacting domains. The N-terminal half shares moderate homology with regions of calmodulin and M-calpain, suggesting that it may also bind calcium. Functional studies indicate that this protein may be an important cofactor for BRCA2 in tumor suppression, and a modulator of CDK2 kinase activity via p21. This protein has also been implicated in the regulation of BRCA2 and RAD51 nuclear focus formation, double-strand break-induced homologous recombination, and cell cycle progression. Multiple transcript variants encoding different isoforms have been described for this gene.

Immunogen: E.coli-derived human BCCIP recombinant protein (Position: K167-K300).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Liu, J., Yuan, Y., Huan, J., Shen, Z. Inhibition of breast and brain cancer cell growth by BCCIP-alpha, an evolutionarily conserved nuclear protein that interacts with BRCA2. Oncogene 20: 336-345, 2001. 2. Lu, H., Yue, J., Meng, X., Nickoloff, J. A., Shen, Z. BCCIP regulates homologous recombination by distinct domains and suppresses spontaneous DNA damage. Nucleic Acids Res. 35: 7160-7170, 2007. 3. Meng, X., Liu, J., Shen, Z. Genomic structure of the human BCCIP gene and its expression in cancer. Gene 302: 139-146, 2003.

Uniprot ID: Q9P287

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross reactivity with other proteins.

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 45 kDa

Calculated Molecular Weight: 52588 MW

Gene ID: 56647

Protein Name: BRCA2 and CDKN1A-interacting protein

Gene Full Name: BRCA2 and CDKN1A interacting protein

Synonyms: BCCIP; Protein TOK 1; TOK 1; TOK1