Anti-ATP5F1A Antibody Picoband®

SKU: A32267-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10^6 cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: ATP5F1A

Specificity:

Background: ATP synthase F1 subunit alpha, mitochondrial is an enzyme that in humans is encoded by the ATP5F1A gene. This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, using an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the alpha subunit of the catalytic core. Alternatively spliced transcript variants encoding the different isoforms have been identified. Pseudogenes of this gene are located on chromosomes 9, 2, and 16.

Immunogen: E.coli-derived human ATP5F1A recombinant protein (Position: R12-Q520).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Akiyama, S., Endo, H., Inohara, N., Ohta, S., Kagawa, Y. Gene structure and cell type-specific expression of the human ATP synthase alpha subunit. Biochim. Biophys. Acta 1219: 129-140, 1994. 2. Baran, A. A., Silverman, K. A., Zeskand, J., Koratkar, R., Palmer, A., McCullen, K., Curran, W. J., Jr., Edmonston, T. B., Siracusa, L. D., Buchberg, A. M. The modifier of Min 2 (Mom2) locus: embryonic lethality of a mutation in the Atp5a1 gene suggests a novel mechanism of polyp suppression. Genome Res. 17: 566-576, 2007. 3. Godbout, R., Pandita, A., Beatty, B., Bie, W., Squire, J. A. Comparative genomic hybridization analysis of Y79 and FISH mapping indicate the amplified human mitochondrial ATP synthase alpha-subunit gene (ATP5A) maps to chromosome 18q12-q21. Cytogenet. Cell Genet. 77: 253-256, 1997.

Uniprot ID: P25705

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 54 kDa

Calculated Molecular Weight: 14377 MW

Gene ID: 498

Protein Name: ATP synthase F

Gene Full Name: ATP synthase F1 subunit alpha

Synonyms: ATP5A; ATP5A1; ATP5AL2; ATPM; Cell and organelle markers; hATP1; Mitochondria Marker; Mitochondrial Marker; Mitochondrion Marker; MOM2; OMR; ORM